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SLFN11 Polyclonal antibody

SLFN11 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26060-1-AP

Print datasheet

Synonyms

Schlafen family member 11, EC:3.1.-.-

验证数据展示

  • WB analysis using 26060-1-AP

    WB analysis using 26060-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26060-1-AP (SLFN11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 26060-1-AP

    IF Staining of A431 using 26060-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using 26060-1-AP (SLFN11 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HEK-293 using 26060-1-AP

    IP experiment of HEK-293 using 26060-1-AP

    IP result of anti-SLFN11 (IP:26060-1-AP, 4ug; Detection:26060-1-AP 1:800) with HEK-293 cells lysate 1610 ug.

  • IHC staining of human ovary cancer using 26060-1-AP

    IHC staining of human ovary cancer using 26060-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26060-1-AP (SLFN11 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inDU 145 cells, HEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

26060-1-AP targets SLFN11 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SLFN11 fusion protein Ag22030 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name schlafen family member 11
Synonyms Schlafen family member 11, EC:3.1.-.-
Calculated Molecular Weight 901 aa, 103 kDa
Observed Molecular Weight 103 kDa
GenBank Accession NumberBC052586
Gene Symbol SLFN11
Gene ID (NCBI) 91607
RRIDAB_2880356
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z7L1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLFN11 antibody 26060-1-APDownload protocol
IHC protocol for SLFN11 antibody 26060-1-APDownload protocol
IF protocol for SLFN11 antibody 26060-1-APDownload protocol
IP protocol for SLFN11 antibody 26060-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Macromol

Electrochemical immunosensor for the predictive cancer biomarker SLFN11 using reduced graphene oxide/MIL-101(Cr)-NH2 composite

Authors - Pandiyaraj Kanagavalli
View Article