• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

SLITRK3 Polyclonal antibody

SLITRK3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 21649-1-AP

Print datasheet

Synonyms

SLIT and NTRK-like protein 3, KIAA0848

验证数据展示

  • WB analysis of mouse brain using 21649-1-AP

    WB analysis of mouse brain using 21649-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 21649-1-AP

    IP experiment of mouse brain using 21649-1-AP

    IP result of anti-SLITRK3 (IP:21649-1-AP, 4ug; Detection:21649-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • IHC staining of mouse brain using 21649-1-AP

    IHC staining of mouse brain using 21649-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse intestine using 21649-1-AP

    IHC staining of mouse intestine using 21649-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse intestine using 21649-1-AP

    IHC staining of mouse intestine using 21649-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat small intestine using 21649-1-AP

    IHC staining of rat small intestine using 21649-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat small intestine using 21649-1-AP

    IHC staining of rat small intestine using 21649-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 21649-1-AP (SLITRK3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat small intestine tissue, mouse brain tissue, mouse intestine
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21649-1-AP targets SLITRK3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen SLITRK3 fusion protein Ag16352 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SLIT and NTRK-like family, member 3
Synonyms SLIT and NTRK-like protein 3, KIAA0848
Calculated Molecular Weight 977 aa, 109 kDa
Observed Molecular Weight 120-125 kDa
GenBank Accession NumberBC114621
Gene Symbol SLITRK3
Gene ID (NCBI) 22865
RRIDAB_10859788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94933
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLITRK3 antibody 21649-1-APDownload protocol
IHC protocol for SLITRK3 antibody 21649-1-APDownload protocol
IP protocol for SLITRK3 antibody 21649-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB, IHC

Neuron

Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.

Authors - Jun Li
View Article
ratWB

Proc Natl Acad Sci U S A

Slitrks control excitatory and inhibitory synapse formation with LAR receptor protein tyrosine phosphatases.

Authors - Yim Yeong Shin YS
  • KD Validated
View Article
humanWB,IF

Mol Biomed

Role of novel cancer gene SLITRK3 to activate NTRK3 in squamous cell lung cancer.

Authors - Aliccia Bollig-Fischer
  • KD Validated
View Article
humanWB

Front Mol Neurosci

Human mutations in SLITRK3 implicated in GABAergic synapse development in mice

Authors - Stephanie Efthymiou
View Article