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SLITRK5 Polyclonal antibody

SLITRK5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21496-1-AP

Print datasheet

Synonyms

SLIT and NTRK like protein 5, LRRC11, Leucine-rich repeat-containing protein 11, KIAA0918, FLJ58374

验证数据展示

  • WB analysis using 21496-1-AP

    WB analysis using 21496-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21496-1-AP (SLITRK5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 21496-1-AP

    IF Staining of A431 using 21496-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SLITRK5 antibody (21496-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21496-1-AP targets SLITRK5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SLITRK5 fusion protein Ag15880 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SLIT and NTRK-like family, member 5
Synonyms SLIT and NTRK like protein 5, LRRC11, Leucine-rich repeat-containing protein 11, KIAA0918, FLJ58374
Calculated Molecular Weight 958 aa, 107 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC098106
Gene Symbol SLITRK5
Gene ID (NCBI) 26050
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94991
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLITRK5 (NTRK-like protein-5), a neuron-specific transmembrane protein, acts as a negative regulator of hedgehog signaling in osteoblasts. SLITRK5 is selectively expressed in osteoblasts and loss of SLITRK5 enhanced osteoblast differentiation in vitro and in vivo. It is a therapeutic target to enhance bone formation (PMID: 34326333). SLITRK5 deficiency impairs corticostriatal circuitry and leads to obsessive-compulsive-like behaviors in mice (PMID: 20418887).

实验方案

Product Specific Protocols
WB protocol for SLITRK5 antibody 21496-1-APDownload protocol
IF protocol for SLITRK5 antibody 21496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols