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  • Featured Product
  • KD/KO Validated

SLU7 Polyclonal antibody

SLU7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 12050-1-AP

Print datasheet

Synonyms

9G8, hSlu7, Pre mRNA splicing factor SLU7, SLU7

验证数据展示

  • WB analysis of Jurkat using 12050-1-AP

    WB analysis of Jurkat using 12050-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 12050-1-AP

    IHC staining of human colon cancer using 12050-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 12050-1-AP (SLU7 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SLU7 antibody included
  • WB analysis using 12050-1-AP

    WB analysis using 12050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12050-1-AP (SLU7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 12050-1-AP

    IHC staining of mouse pancreas using 12050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLU7 antibody included
  • IHC staining of mouse pancreas using 12050-1-AP

    IHC staining of mouse pancreas using 12050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLU7 antibody included
  • IHC staining of rat pancreas using 12050-1-AP

    IHC staining of rat pancreas using 12050-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLU7 antibody included
  • IHC staining of rat stomach using 12050-1-AP

    IHC staining of rat stomach using 12050-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 12050-1-AP (SLU7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SLU7 antibody included

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, mouse colon tissue, K-562 cells, Y79 cells
Positive IHC detected inmouse pancreas tissue, human colon cancer tissue, rat pancreas tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SLU7 Ready-To-Use IHC Kit. SLU7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12050-1-AP targets SLU7 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, RIP, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SLU7 fusion protein Ag2680 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SLU7 splicing factor homolog (S. cerevisiae)
Synonyms 9G8, hSlu7, Pre mRNA splicing factor SLU7, SLU7
Calculated Molecular Weight 586 aa, 68 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC010634
Gene Symbol SLU7
Gene ID (NCBI) 10569
RRIDAB_10663102
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95391
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pre-mRNA splicing occurs in two sequential transesterification steps. SLU7 is a splicing factor that has been found to be essential during the second catalytic step in the pre-mRNA splicing process. It associates with the spliceosome and contains a zinc knuckle motif that is found in other splicing factors and is involved in protein-nucleic acid and protein-protein interactions.

实验方案

Product Specific Protocols
WB protocol for SLU7 antibody 12050-1-APDownload protocol
IHC protocol for SLU7 antibody 12050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanRIP

Mol Cancer

A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer

Authors - Runjie Song
View Article
human,mouseWB,IHC,IF

Adv Sci (Weinh)

G3BP1 and SLU7 Jointly Promote Immune Evasion by Downregulating MHC-I via PI3K/Akt Activation in Bladder Cancer

Authors - Xianchong Zheng
  • KD Validated
View Article