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验证数据展示

  • IHC staining of human liver cancer using 67343-1-Ig (same clone as 67343-1-PBS)

    IHC staining of human liver cancer using 67343-1-Ig (same clone as 67343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67343-1-Ig (SMAD2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD2 antibody included
  • IHC staining of human liver cancer using 67343-1-Ig (same clone as 67343-1-PBS)

    IHC staining of human liver cancer using 67343-1-Ig (same clone as 67343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67343-1-Ig (SMAD2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD2 antibody included
  • IF Staining of HepG2 using 67343-1-Ig (same clone as 67343-1-PBS)

    IF Staining of HepG2 using 67343-1-Ig (same clone as 67343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67343-1-Ig (SMAD2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

  • WB analysis using 67343-1-Ig (same clone as 67343-1-PBS)

    WB analysis using 67343-1-Ig (same clone as 67343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67343-1-Ig (SMAD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

SMAD2 Monoclonal antibody, PBS Only

SMAD2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67343-1-PBS

产品说明书

CloneNo. 2H10C7

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

hMAD 2, hSMAD2, JV18, JV18 1, MAD homolog 2, Mad related protein 2, MADH2, MADR2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SMAD2 Ready-To-Use IHC Kit. SMAD2 primary antibody included.

产品信息

67343-1-PBS targets SMAD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 SMAD2 fusion protein Ag19542 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SMAD family member 2
别名 hMAD 2, hSMAD2, JV18, JV18 1, MAD homolog 2, Mad related protein 2, MADH2, MADR2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD2
计算分子量 467 aa, 52 kDa
观测分子量 58 kDa
GenBank蛋白编号BC014840
基因名称 SMAD2
Gene ID (NCBI) 4087
RRIDAB_2882601
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ15796
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)