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SMAD2 Monoclonal antibody, PBS Only

SMAD2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H10C7

Cat no : 67343-1-PBS

Print datasheet

Synonyms

hMAD 2, hSMAD2, JV18, JV18 1, MAD homolog 2, Mad related protein 2, MADH2, MADR2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD2

验证数据展示

  • IHC staining of human liver cancer using 67343-1-Ig (same clone as 67343-1-PBS)

    IHC staining of human liver cancer using 67343-1-Ig (same clone as 67343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67343-1-Ig (SMAD2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD2 antibody included
  • IHC staining of human liver cancer using 67343-1-Ig (same clone as 67343-1-PBS)

    IHC staining of human liver cancer using 67343-1-Ig (same clone as 67343-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67343-1-Ig (SMAD2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD2 antibody included
  • IF Staining of HepG2 using 67343-1-Ig (same clone as 67343-1-PBS)

    IF Staining of HepG2 using 67343-1-Ig (same clone as 67343-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67343-1-Ig (SMAD2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

  • WB analysis using 67343-1-Ig (same clone as 67343-1-PBS)

    WB analysis using 67343-1-Ig (same clone as 67343-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67343-1-Ig (SMAD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67343-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SMAD2 Ready-To-Use IHC Kit. SMAD2 primary antibody included.

产品信息

67343-1-PBS targets SMAD2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen SMAD2 fusion protein Ag19542 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SMAD family member 2
Synonyms hMAD 2, hSMAD2, JV18, JV18 1, MAD homolog 2, Mad related protein 2, MADH2, MADR2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD2
Calculated Molecular Weight 467 aa, 52 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC014840
Gene Symbol SMAD2
Gene ID (NCBI) 4087
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15796
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)