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SMAD3 Polyclonal antibody

SMAD3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30130-1-AP

Print datasheet

Synonyms

DKFZp586N0721, DKFZp686J10186, hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3

验证数据展示

  • WB analysis using 30130-1-AP

    WB analysis using 30130-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30130-1-AP (SMAD3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30130-1-AP targets SMAD3 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen SMAD3 fusion protein Ag32595 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SMAD family member 3
Synonyms DKFZp586N0721, DKFZp686J10186, hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3
Calculated Molecular Weight48kd
Observed Molecular Weight48-55 kDa
GenBank Accession NumberNM_005902
Gene Symbol SMAD3
Gene ID (NCBI) 4088
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP84022
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

实验方案

Product Specific Protocols
WB protocol for SMAD3 antibody 30130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols