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验证数据展示

  • WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

    WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 66516-1-Ig (same clone as 66516-1-PBS)

    IHC staining of human stomach cancer using 66516-1-Ig (same clone as 66516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD3 antibody included
  • IHC staining of mouse lung using 66516-1-Ig (same clone as 66516-1-PBS)

    IHC staining of mouse lung using 66516-1-Ig (same clone as 66516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD3 antibody included

SMAD3 Monoclonal antibody, PBS Only

SMAD3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66516-1-PBS

产品说明书

CloneNo. 1F10B2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SMAD3 Ready-To-Use IHC Kit. SMAD3 primary antibody included.

产品信息

66516-1-PBS targets SMAD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SMAD family member 3
别名 JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3
计算分子量 48 kDa
观测分子量 55-60 kDa
GenBank蛋白编号NM_005902
基因名称 SMAD3
Gene ID (NCBI) 4088
RRIDAB_2881879
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP84022
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.