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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

    WB analysis using 66516-1-Ig (same clone as 66516-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 66516-1-Ig (same clone as 66516-1-PBS)

    IHC staining of human stomach cancer using 66516-1-Ig (same clone as 66516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD3 antibody included
  • IHC staining of mouse lung using 66516-1-Ig (same clone as 66516-1-PBS)

    IHC staining of mouse lung using 66516-1-Ig (same clone as 66516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 66516-1-Ig (SMAD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66516-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD3 antibody included

SMAD3 Monoclonal antibody, PBS Only

SMAD3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66516-1-PBS

Print datasheet

CloneNo. 1F10B2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SMAD3 Ready-To-Use IHC Kit. SMAD3 primary antibody included.

产品信息

66516-1-PBS targets SMAD3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SMAD family member 3
Synonyms JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberNM_005902
Gene Symbol SMAD3
Gene ID (NCBI) 4088
RRIDAB_2881879
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP84022
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.