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SMAD4 Polyclonal antibody

SMAD4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

Cat no : 51069-2-AP

Print datasheet

Synonyms

DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

验证数据展示

  • WB analysis using 51069-2-AP

    WB analysis using 51069-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51069-2-AP (SMAD4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 51069-2-AP

    IHC staining of human tonsillitis using 51069-2-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 51069-2-AP (SMAD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMAD4 antibody included
  • WB analysis of HepG2 cells using 51069-2-AP

    WB analysis of HepG2 cells using 51069-2-AP

    WB result of SMAD4 antibody (51069-1-AP, 1:1000) with sh-coontrol and sh-SMAD4 transfected HepG2 cells.

  • IF Staining of HepG2 using 51069-2-AP

    IF Staining of HepG2 using 51069-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMAD4 antibody (51069-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 51069-2-AP

    FC experiment of HepG2 using 51069-2-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human SMAD4 (51069-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, COLO 320 cells, PC-3 cells, HEK-293 cells, A431 cells, A549 cells, MCF-7 cells
Positive IHC detected inhuman tonsillitis tissue, human lung cancer tissue, human heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SMAD4 Ready-To-Use IHC Kit. SMAD4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51069-2-AP targets SMAD4 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SMAD family member 4
Synonyms DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC002379
Gene Symbol SMAD4
Gene ID (NCBI) 4089
RRIDAB_2193321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13485
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human SMAD4.

实验方案

Product Specific Protocols
WB protocol for SMAD4 antibody 51069-2-APDownload protocol
IHC protocol for SMAD4 antibody 51069-2-APDownload protocol
IF protocol for SMAD4 antibody 51069-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
rat,mouseIF

Glia

αV integrins in Schwann cells promote attachment to axons, but are dispensable in vivo.

Authors - Kathleen K Catignas
View Article
humanWB

Chemosphere

PFOS-induced placental cell growth inhibition is partially mediated by lncRNA H19 through interacting with miR-19a and miR-19b.

Authors - Jing Li
View Article
mouseWB,IF

Biochim Biophys Acta Mol Basis Dis

Deficiency of M-LP/Mpv17L leads to development of β-cell hyperplasia and improved glucose tolerance via activation of the Wnt and TGF-β pathways.

Authors - Reiko Iida
View Article
mouseWB,IF

Physiol Res

Fluid Shear Stress-Induced Down-Regulation of miR-146a-5p Inhibits Osteoblast Apoptosis via Targeting SMAD4

Authors - X Liu
View Article