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SMAD4 Recombinant antibody, PBS Only (Detector)

SMAD4 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240026D10

Cat no : 83169-2-PBS

Print datasheet

Synonyms

MADH4, MAD homolog 4, JIP, hSMAD4, DPC4

验证数据展示

  • Sandwich ELISA standard curve of MP00213-3

    Sandwich ELISA standard curve of MP00213-3

    Sandwich ELISA standard curve of MP00213-3, Rat KIM-1/HAVCR1 Monoclonal Matched Antibody Pair - PBS only. 83221-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0597. 83169-2-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00213-3

    Sample test of MP00213-3

    Organs from 2 rats were rinsed with PBS to remove excess blood, chopped into 1-2 mm pieces, homogenized in 5-10 mL of PBS in a tissue homogenizer, and stored at ≤ -80℃ 5 min. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g to remove particulate. Homogenates from rat kideny tissue were assayed for rat KIM-1/HAVCR1 and measured 71.6 pg/mL.

  • Sample test of MP00213-3

    Sample test of MP00213-3

    Serum of eight individual healthy rats were measured. The rat KIM-1/HAVCR1 concentration of detected samples was determined to be 478.5 pg/mL with a range of 105.6-1,038.5 pg/mL.

  • Cytometric bead array standard curve of MP00267-1

    Cytometric bead array standard curve of MP00267-1

    Cytometric bead array standard curve of MP00267-1, SMAD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83169-4-PBS. Detection antibody: 83169-2-PBS. Standard: Ag0299. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00267-3

    Cytometric bead array standard curve of MP00267-3

    Cytometric bead array standard curve of MP00267-3, SMAD4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83169-3-PBS. Detection antibody: 83169-2-PBS. Standard: Ag0299. Range: 0.625-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy SMAD4 Ready-To-Use IHC Kit. SMAD4 primary antibody included.

产品信息

83169-2-PBS targets SMAD4 as part of a matched antibody pair:

MP00213-3: 83221-1-PBS capture and 83169-2-PBS detection (validated in Sandwich ELISA)

MP00267-1: 83169-4-PBS capture and 83169-2-PBS detection (validated in Cytometric bead array)

MP00267-3: 83169-3-PBS capture and 83169-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen SMAD4 fusion protein Ag0299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SMAD family member 4
Synonyms MADH4, MAD homolog 4, JIP, hSMAD4, DPC4
Calculated Molecular Weight 60 kDa
GenBank Accession NumberBC002379
Gene Symbol SMAD4
Gene ID (NCBI) 4089
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13485
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C