SMAD7 Monoclonal antibody
SMAD7 Monoclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, pig and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2B9A4
验证数据展示
经过测试的应用
Positive WB detected in | pig brain tissue, pig kidney tissue, rat brain tissue, rat kidney tissue, mouse brain tissue, mouse skeletal muscle tissue |
Positive IHC detected in | mouse cerebellum tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 9 publications below |
产品信息
66478-1-Ig targets SMAD7 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
Tested Applications | WB, IF, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, mouse, rat, pig |
Cited Reactivity | human, mouse, rat |
Immunogen | SMAD7 fusion protein Ag13688 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | SMAD family member 7 |
Synonyms | CRCS3, hSMAD7, MAD homolog 7, MAD homolog 8, MADH7, MADH8, Mothers against DPP homolog 7, Mothers against DPP homolog 8, SMAD 7, SMAD family member 7, SMAD7 |
Calculated Molecular Weight | 426 aa, 46 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC074819 |
Gene Symbol | SMAD7 |
Gene ID (NCBI) | 4092 |
RRID | AB_2881844 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O15105 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SMAD7 antibody 66478-1-Ig | Download protocol |
IHC protocol for SMAD7 antibody 66478-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Pharmacol Sin The multifunctional adaptor protein HIP-55 couples Smad7 to accelerate TGF-β type I receptor degradation.
| ||
Aging (Albany NY) LINC00968 can inhibit the progression of lung adenocarcinoma through the miR-21-5p/SMAD7 signal axis. | ||
Biomed Pharmacother Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity. | ||
J Ethnopharmacol Wu-Mei-Wan ameliorates chronic colitis-associated intestinal fibrosis through inhibiting fibroblast activation. | ||
Exp Cell Res Exosomes derived from thymic stromal lymphopoietin-treated dendritic cells regulate T helper 17/regulatory T cell differentiation via miR-21/Smad7 axis. | ||
Biochem Biophys Res Commun Emodin ameliorates tubulointerstitial fibrosis in obstructed kidneys by inhibiting EZH2. |