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SMAP1 Polyclonal antibody

SMAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12799-1-AP

Print datasheet

Synonyms

Stromal membrane-associated protein 1, SMAP 1

验证数据展示

  • WB analysis of NIH/3T3 using 12799-1-AP

    WB analysis of NIH/3T3 using 12799-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12799-1-AP (SMAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 12799-1-AP

    IHC staining of human stomach using 12799-1-AP

    Immunohistochemical analysis of paraffin-embedded normal stomach slide using 12799-1-AP (SMAP1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNIH/3T3 cells, human adrenal gland tissue, human plasma, mouse brain tissue, rat lymph tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12799-1-AP targets SMAP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SMAP1 fusion protein Ag3544 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small ArfGAP 1
Synonyms Stromal membrane-associated protein 1, SMAP 1
Calculated Molecular Weight 467 aa, 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC036123
Gene Symbol SMAP1
Gene ID (NCBI) 60682
RRIDAB_2272651
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYB5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMAP1, also named as FLJ13159 and SMAP 1, contains a Arf-GAP domain. SMAP1 is a GTPase activating protein for ARF6 that binds clathrin and regulates clathrin-dependent endocytosis. SMAP1 may play a role in erythropoiesis. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SMAP1.

实验方案

Product Specific Protocols
WB protocol for SMAP1 antibody 12799-1-APDownload protocol
IHC protocol for SMAP1 antibody 12799-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols