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验证数据展示

  • WB analysis of HeLa using 20650-1-AP

    WB analysis of HeLa using 20650-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 20650-1-AP

    WB analysis of HepG2 using 20650-1-AP

    WB result of SMARCA4/BRG1 antibody (20650-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells.

  • Featured Product
  • KD/KO验证

SMARCA4/BRG1 Polyclonal antibody

SMARCA4/BRG1 Polyclonal Antibody for WB, ELISA
Cat No. 20650-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA and More (2)

别名

SMARCA4, BRG1 associated factor 190A, BRG1, BAF190A, BAF190

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SMARCA4 Ready-To-Use IHC Kit. SMARCA4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20650-1-AP targets SMARCA4/BRG1 in WB, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IF, ChIP
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
别名 SMARCA4, BRG1 associated factor 190A, BRG1, BAF190A, BAF190
计算分子量 185 kDa
观测分子量 180 kDa
GenBank蛋白编号NM_001128844
基因名称 SMARCA4
Gene ID (NCBI) 6597
RRIDAB_10694827
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51532
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to SMARCA4.

实验方案

Product Specific Protocols
WB protocol for SMARCA4/BRG1 antibody 20650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF,ChIP

Acta Pharmacol Sin

Cardiac-specific deletion of BRG1 ameliorates ventricular arrhythmia in mice with myocardial infarction

Authors - Jing Li
View Article