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SMARCA4 Recombinant antibody

SMARCA4 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240172F7

Cat no : 83310-7-RR

Print datasheet

Synonyms

BRG1, BAF190A, BAF190, ATP-dependent helicase SMARCA4, ATP dependent helicase SMARCA4

验证数据展示

  • WB analysis using 83310-7-RR

    WB analysis using 83310-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83310-7-RR (SMARCA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 83310-7-RR

    IHC staining of mouse kidney using 83310-7-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCA4 antibody included
  • IHC staining of human colon cancer using 83310-7-RR

    IHC staining of human colon cancer using 83310-7-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCA4 antibody included
  • IHC staining of human colon cancer using 83310-7-RR

    IHC staining of human colon cancer using 83310-7-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83310-7-RR (SMARCA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMARCA4 antibody included
  • FC experiment of Jurkat using 83310-7-RR

    FC experiment of Jurkat using 83310-7-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug SMARCA4 Recombinant antibody (83310-7-RR, Clone:240172F7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83310-7-RR

    Affinity and Kinetic Characterization of 83310-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83310-7-RR against Human SMARCA4 were performed. The affinity constant is 1.73 nM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, HEK-293T cells, C6 cells
Positive IHC detected inhuman colon cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy SMARCA4 Ready-To-Use IHC Kit. SMARCA4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83310-7-RR is SMARCA4 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SMARCA4 fusion protein Ag16256 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms BRG1, BAF190A, BAF190, ATP-dependent helicase SMARCA4, ATP dependent helicase SMARCA4
Calculated Molecular Weight 1647 aa, 185 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC150298
Gene Symbol SMARCA4
Gene ID (NCBI) 6597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP51532
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

实验方案

Product Specific Protocols
WB protocol for SMARCA4 antibody 83310-7-RRDownload protocol
IHC protocol for SMARCA4 antibody 83310-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols