WB Figures
WB analysis using 21634-1-AP (same clone as 21634-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
WB analysis using 21634-1-AP (same clone as 21634-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 21634-1-AP (same clone as 21634-1-PBS)
WB result of SMARCA4/BRG1 antibody (21634-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 21634-1-AP (same clone as 21634-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 21634-1-AP (same clone as 21634-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 21634-1-AP (same clone as 21634-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 21634-1-AP (SMARCA4/BRG1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of human gliomas using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded human gliomas using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieved with Citric acid buffer, pH6.0. This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IHC staining of mouse kidney using 21634-1-AP (same clone as 21634-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21634-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HEK-293 using 21634-1-AP (same clone as 21634-1-PBS)
Immunofluorescent analysis of 293 cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 21634-1-AP (same clone as 21634-1-PBS)
Immunofluorescent analysis of Hela cells, using SMARCA4 antibody 21634-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 21634-1-AP (same clone as 21634-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (21634-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 21634-1-PBS in a different storage buffer formulation.
