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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IP experiment of HeLa using 66561-1-Ig (same clone as 66561-1-PBS)

    IP experiment of HeLa using 66561-1-Ig (same clone as 66561-1-PBS)

    IP result of anti-SMARCA4/BRG1 (IP:66561-1-Ig, 5ug; Detection:66561-1-Ig 1:3000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    WB analysis of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    WB result of SMARCA4/BRG1 antibody (66561-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • WB analysis using 66561-1-Ig (same clone as 66561-1-PBS)

    WB analysis using 66561-1-Ig (same clone as 66561-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66561-1-Ig (SMARCA4/BRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • WB analysis of COLO 320 using 66561-1-Ig (same clone as 66561-1-PBS)

    WB analysis of COLO 320 using 66561-1-Ig (same clone as 66561-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66561-1-Ig (SMARCA4/BRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:50000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 66561-1-Ig (same clone as 66561-1-PBS)

    IF Staining of PC-3 using 66561-1-Ig (same clone as 66561-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:50000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SMARCA4/BRG1 Monoclonal antibody, PBS Only

SMARCA4/BRG1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 66561-1-PBS

产品说明书

CloneNo. 2E6B6

宿主/亚型

Mouse / IgG2b

种属反应性

Human, rat

应用

WB, IF/ICC, IP, ELISA

别名

ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SMARCA4/BRG1, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SMARCA4 Ready-To-Use IHC Kit. SMARCA4 primary antibody included.

产品信息

66561-1-PBS targets SMARCA4/BRG1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, rat
免疫原 SMARCA4/BRG1 fusion protein Ag16256 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
别名 ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SMARCA4/BRG1, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1
计算分子量 1647 aa, 185 kDa
观测分子量 185 kDa
GenBank蛋白编号BC150298
基因名称 SMARCA4
Gene ID (NCBI) 6597
RRIDAB_2881922
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP51532
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.