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  • Featured Product
  • KD/KO Validated

SMARCA4/BRG1 Monoclonal antibody, PBS Only

SMARCA4/BRG1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2E6B6

Cat no : 66561-1-PBS

Print datasheet

Synonyms

ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SMARCA4/BRG1, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1

验证数据展示

  • IP experiment of HeLa using 66561-1-Ig (same clone as 66561-1-PBS)

    IP experiment of HeLa using 66561-1-Ig (same clone as 66561-1-PBS)

    IP result of anti-SMARCA4/BRG1 (IP:66561-1-Ig, 5ug; Detection:66561-1-Ig 1:3000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    WB analysis of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    WB result of SMARCA4/BRG1 antibody (66561-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • WB analysis using 66561-1-Ig (same clone as 66561-1-PBS)

    WB analysis using 66561-1-Ig (same clone as 66561-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66561-1-Ig (SMARCA4/BRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • WB analysis of COLO 320 using 66561-1-Ig (same clone as 66561-1-PBS)

    WB analysis of COLO 320 using 66561-1-Ig (same clone as 66561-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66561-1-Ig (SMARCA4/BRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:50000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 66561-1-Ig (same clone as 66561-1-PBS)

    IF Staining of PC-3 using 66561-1-Ig (same clone as 66561-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:50000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    IF Staining of HepG2 using 66561-1-Ig (same clone as 66561-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMARCA4/BRG1 antibody (66561-1-Ig, Clone: 2E6B6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66561-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SMARCA4 Ready-To-Use IHC Kit. SMARCA4 primary antibody included.

产品信息

66561-1-PBS targets SMARCA4/BRG1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, rat
Immunogen SMARCA4/BRG1 fusion protein Ag16256 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Synonyms ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SMARCA4/BRG1, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1
Calculated Molecular Weight 1647 aa, 185 kDa
Observed Molecular Weight 185 kDa
GenBank Accession NumberBC150298
Gene Symbol SMARCA4
Gene ID (NCBI) 6597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51532
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.