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- KD/KO Validated
SMARCB1 Polyclonal antibody
SMARCB1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, K-562 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human lymphoma tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20654-1-AP targets SMARCB1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB, IF |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 |
Synonyms | BAF47, BRG1 associated factor 47, hSNF5, hSNFS, INI1, INI-1, Integrase interactor 1 protein, RDT, Sfh1p, SMARCB1, SNF5, SNF5 homolog, SNF5L1, Snr1 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 40-45 kDa |
GenBank Accession Number | NM_003073 |
Gene Symbol | SMARCB1 |
Gene ID (NCBI) | 6598 |
RRID | AB_10695761 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q12824 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SMARCB1 antibody 20654-1-AP | Download protocol |
IHC protocol for SMARCB1 antibody 20654-1-AP | Download protocol |
IP protocol for SMARCB1 antibody 20654-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function. | ||
Front Cell Dev Biol Upregulation of Neogenin-1 by a CREB1-BAF47 Complex in Vascular Endothelial Cells is Implicated in Atherogenesis.
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Transl Oncol SNF5, a core subunit of SWI/SNF complex, regulates melanoma cancer cell growth, metastasis, and immune escape in response to matrix stiffness. | ||
Bioengineered SNF5 promotes cell proliferation and immune evasion in non-small cell lung cancer.
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