SMARCC1 Polyclonal antibody
SMARCC1 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, Jurkat cells, rat brain tissue, rat testis tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human malignant melanoma tissue, human appendicitis tissue, human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17722-1-AP targets SMARCC1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | WB, IP, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | SMARCC1 fusion protein Ag12109 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 |
Synonyms | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SRG3, Rsc8, CRACC1, BRG1-associated factor 155 |
Calculated Molecular Weight | 1105 aa, 123 kDa |
Observed Molecular Weight | 155-160 kDa |
GenBank Accession Number | BC050564 |
Gene Symbol | SMARCC1 |
Gene ID (NCBI) | 6599 |
RRID | AB_2191987 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q92922 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SMARCC1 antibody 17722-1-AP | Download protocol |
IHC protocol for SMARCC1 antibody 17722-1-AP | Download protocol |
IP protocol for SMARCC1 antibody 17722-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Discov A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction. | ||
Nucleic Acids Res HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription. | ||
Mol Cell Biol Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex. |