验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, Jurkat cells, rat brain tissue, rat testis tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human malignant melanoma tissue, human appendicitis tissue, human brain tissue, human kidney tissue, human ovary tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17722-1-AP targets SMARCC1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SMARCC1 fusion protein Ag12109 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 |
| 别名 | SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SRG3, Rsc8, CRACC1, BRG1-associated factor 155 |
| 计算分子量 | 1105 aa, 123 kDa |
| 观测分子量 | 155-160 kDa |
| GenBank蛋白编号 | BC050564 |
| 基因名称 | SMARCC1 |
| Gene ID (NCBI) | 6599 |
| RRID | AB_2191987 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92922 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMARCC1, also named as SWI/SNF complex 155 kDa subunit or BAF155, is a 1105 amino acid protein, which belongs to the SMARCC family. SMARCC1 is expressed in brain, heart, muscle, placenta, lung, liver, muscle, kidney and pancreas. SMARCC1 is Involved in transcriptional activation and repression of select genes by chromatin remodeling. SMARCC1 may stimulate the ATPase activity of the catalytic subunit of the complex. SMARCC1 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calculated molecular weight of TCEA1 is 123 kDa, but modified SMARCC1 is about 155-160 kDa. (PMID: 24025402)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMARCC1 antibody 17722-1-AP | Download protocol |
| IHC protocol for SMARCC1 antibody 17722-1-AP | Download protocol |
| IP protocol for SMARCC1 antibody 17722-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Discov A role for SMARCB1 in synovial sarcomagenesis reveals that SS18-SSX induces canonical BAF destruction. | ||
Nucleic Acids Res HRP2-DPF3a-BAF complex coordinates histone modification and chromatin remodeling to regulate myogenic gene transcription. | ||
Mol Cell Biol Growth Factor Independence 1B-Mediated Transcriptional Repression and Lineage Allocation Require Lysine-Specific Demethylase 1-Dependent Recruitment of the BHC Complex. |
