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验证数据展示

  • WB analysis of HeLa using 12018-1-AP

    WB analysis of HeLa using 12018-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human medulloblastoma using 12018-1-AP

    IHC staining of human medulloblastoma using 12018-1-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12018-1-AP (SMARCC2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SMARCC2 antibody included
  • IP experiment of HeLa using 12018-1-AP

    IP experiment of HeLa using 12018-1-AP

    IP result of anti-BAF170 (IP:12018-1-AP, 4ug; Detection:12018-1-AP 1:700) with HeLa cells lysate 3200ug.

  • IF Staining of HepG2 using 12018-1-AP

    IF Staining of HepG2 using 12018-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BAF170 antibody (12018-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, green).

BAF170 Polyclonal antibody

BAF170 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12018-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SMARCC2, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2, Rsc8, CRACC2, BRG1-associated factor 170

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A431 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman medulloblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SMARCC2 Ready-To-Use IHC Kit. SMARCC2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12018-1-AP targets BAF170 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen BAF170 fusion protein Ag2634 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
Synonyms SMARCC2, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2, Rsc8, CRACC2, BRG1-associated factor 170
Calculated Molecular Weight 1214 aa, 132 kDa
Observed Molecular Weight 170 kDa
GenBank Accession NumberBC013045
Gene Symbol BAF170
Gene ID (NCBI) 6601
RRIDAB_2192010
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAQ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BAF170, also named as SWI/SNF complex 170 kDa subunit or SMARCC2, is a 1214 amino acid protein, which belongs to the SMARCC family. BAF170 is ubiquitously expressed. BAF170 is involved in transcriptional activation and repression of select genes by chromatin remodeling. BAF170 may be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. BAF170 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calcualted molecular weight of BAF170, is 133 kDa, but modified BAF170 is about 170 kDa. (PMID: 8804307)

实验方案

Product Specific Protocols
WB protocol for BAF170 antibody 12018-1-APDownload protocol
IHC protocol for BAF170 antibody 12018-1-APDownload protocol
IF protocol for BAF170 antibody 12018-1-APDownload protocol
IP protocol for BAF170 antibody 12018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Cell

SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.

Authors - Shibin Hu
View Article
humanWB,IP

Aging (Albany NY)

FBXO28 promotes proliferation, invasion, and metastasis of pancreatic cancer cells through regulation of SMARCC2 ubiquitination

Authors - Songbai Liu
View Article
humanWB

Biochem Biophys Res Commun

SMARCC2 silencing suppresses oncogenic activation through modulation of chromatin accessibility in breast cancer

Authors - Zhaoran Sun
  • KD Validated
View Article