SMARCE1 Polyclonal antibody
SMARCE1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, MCF-7 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
10814-1-AP targets SMARCE1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IP, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | mouse |
Immunogen | SMARCE1 fusion protein Ag1161 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 |
Synonyms | BAF57, BRG1 associated factor 57, SMARCE1 |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC007082 |
Gene Symbol | SMARCE1 |
Gene ID (NCBI) | 6605 |
RRID | AB_2192174 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q969G3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SMARCE1 antibody 10814-1-AP | Download protocol |
IHC protocol for SMARCE1 antibody 10814-1-AP | Download protocol |
IP protocol for SMARCE1 antibody 10814-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
iScience WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation |