验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
10814-1-AP targets SMARCE1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Immunogen | SMARCE1 fusion protein Ag1161 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1 |
| Synonyms | BAF57, BRG1 associated factor 57, SMARCE1 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC007082 |
| Gene Symbol | SMARCE1 |
| Gene ID (NCBI) | 6605 |
| RRID | AB_2192174 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969G3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Via antagonizing chromatin-mediated transcriptional repression, the SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes. The BRG1/brm-associated factors, or SMARCE1, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF subunits. It contains a DNA-binding HMG domain and performs multiple biological functions, including DNA-, protein- and chromatin-binding, transcription coactivating activity, chromatin modification, DNA-dependent negative regulation of transcription, regulation of transcription of the RNA polymerase II promoter. In addition, SMARCE1, as an ER (estrogen receptor) subtype-selective modulator, regulates ER mediated transcription in a ligand-dependent manner and also directly binds to AR (androgen receptor) to alter its activity. The calculated molecular weight of SMARCE1 is 47 kDa, but modified SMARCE1 is about 55 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMARCE1 antibody 10814-1-AP | Download protocol |
| IHC protocol for SMARCE1 antibody 10814-1-AP | Download protocol |
| IP protocol for SMARCE1 antibody 10814-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
