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SMC2 Polyclonal antibody

SMC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30707-1-AP

Print datasheet

Synonyms

SMC2L1, SMC protein 2, SMC 2, hCAP E, CAPE

验证数据展示

  • WB analysis using 30707-1-AP

    WB analysis using 30707-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30707-1-AP (SMC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 30707-1-AP

    IHC staining of human ovary cancer using 30707-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30707-1-AP (SMC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMC2 antibody included
  • IHC staining of human ovary cancer using 30707-1-AP

    IHC staining of human ovary cancer using 30707-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30707-1-AP (SMC2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SMC2 antibody included
  • IF Staining of HeLa using 30707-1-AP

    IF Staining of HeLa using 30707-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMC2 antibody (30707-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, Raji cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SMC2 Ready-To-Use IHC Kit. SMC2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30707-1-AP targets SMC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen SMC2 fusion protein Ag33471 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name structural maintenance of chromosomes 2
Synonyms SMC2L1, SMC protein 2, SMC 2, hCAP E, CAPE
Calculated Molecular Weight136 kDa
Observed Molecular Weight 135 kDa
GenBank Accession NumberNM_001042550.1
Gene Symbol SMC2
Gene ID (NCBI) 10592
RRIDAB_3086394
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95347
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMC2 is a central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (PMID: 11136719).

实验方案

Product Specific Protocols
WB protocol for SMC2 antibody 30707-1-APDownload protocol
IHC protocol for SMC2 antibody 30707-1-APDownload protocol
IF protocol for SMC2 antibody 30707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols