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SMC4 Polyclonal antibody
SMC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells |
Positive IHC detected in | human colon tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
24758-1-AP targets SMC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat |
Immunogen | SMC4 fusion protein Ag19156 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | structural maintenance of chromosomes 4 |
Synonyms | hCAP-C, hCAPC, hCAP C, Chromosome-associated polypeptide C, CAPC |
Calculated Molecular Weight | 1288 aa, 147 kDa |
Observed Molecular Weight | 147-150 kDa |
GenBank Accession Number | BC106033 |
Gene Symbol | SMC4 |
Gene ID (NCBI) | 10051 |
RRID | AB_2879707 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NTJ3 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SMC4 antibody 24758-1-AP | Download protocol |
IHC protocol for SMC4 antibody 24758-1-AP | Download protocol |
IF protocol for SMC4 antibody 24758-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Hum Mol Genet Single-cell transcription profiles in Bloom syndrome patients link BLM deficiency with altered condensin complex expression signatures. | ||
Gene Transcriptional profiling of rat skeletal muscle hypertrophy under restriction of blood flow. | ||
Sci Rep Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes. | ||
Cell Metab The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity
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