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SMC4 Polyclonal antibody

SMC4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 24758-1-AP

Print datasheet

Synonyms

hCAP-C, hCAPC, hCAP C, Chromosome-associated polypeptide C, CAPC



经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman colon tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SMC4 Ready-To-Use IHC Kit. SMC4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24758-1-AP targets SMC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen SMC4 fusion protein Ag19156 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name structural maintenance of chromosomes 4
Synonyms hCAP-C, hCAPC, hCAP C, Chromosome-associated polypeptide C, CAPC
Calculated Molecular Weight 1288 aa, 147 kDa
Observed Molecular Weight 147-150 kDa
GenBank Accession NumberBC106033
Gene Symbol SMC4
Gene ID (NCBI) 10051
RRIDAB_2879707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NTJ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMC4, also named as Structural maintenance of chromosomes protein 4, is a 1288 amino acid protein, which is widely expressed and belongs to the SMC family, SMC4 subfamily. SMC4 is a Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The calculated molecular weight of SMC4 is 147 kDa, but modified SMC4 is about 160 kDa.

实验方案

Product Specific Protocols
WB protocol for SMC4 antibody 24758-1-APDownload protocol
IHC protocol for SMC4 antibody 24758-1-APDownload protocol
IF protocol for SMC4 antibody 24758-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Single-cell transcription profiles in Bloom syndrome patients link BLM deficiency with altered condensin complex expression signatures.

Authors - Ipek Ilgin Gönenc
ratIHC

Gene

Transcriptional profiling of rat skeletal muscle hypertrophy under restriction of blood flow.

Authors - Shouyu Xu

Sci Rep

Cryo-Electron Tomography and Proteomics studies of centrosomes from differentiated quiescent thymocytes.

Authors - Johan Busselez
human,mouseWB,IHC,IF

Cell Metab

The diapause-like colorectal cancer cells induced by SMC4 attenuation are characterized by low proliferation and chemotherapy insensitivity

Authors - Xuedan Sun
  • KO Validated