验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse testis tissue, NIH/3T3 cells, rat testis tissue |
| Positive IP detected in | HeLa cells, mouse testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
14465-1-AP targets SMC6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | SMC6 fusion protein Ag5812 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | structural maintenance of chromosomes 6 |
| Synonyms | hSMC6, SMC 6, SMC protein 6, SMC6, SMC6L1 |
| Calculated Molecular Weight | 126 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC039828 |
| Gene Symbol | SMC6 |
| Gene ID (NCBI) | 79677 |
| RRID | AB_2192792 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96SB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMC6 antibody 14465-1-AP | Download protocol |
| IHC protocol for SMC6 antibody 14465-1-AP | Download protocol |
| IP protocol for SMC6 antibody 14465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
