- Featured Product
- KD/KO Validated
SMCR7/MID49 Polyclonal antibody
SMCR7/MID49 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HepG2 cells, HT-1080 cells |
| Positive IHC detected in | human liver cancer tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16413-1-AP targets SMCR7/MID49 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, monkey |
| Immunogen | SMCR7/MID49 fusion protein Ag9531 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | Smith-Magenis syndrome chromosome region, candidate 7 |
| Synonyms | MID49, MIEF2, SMCR7 |
| Calculated Molecular Weight | 454 aa, 49 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC014973 |
| Gene Symbol | MID49 |
| Gene ID (NCBI) | 125170 |
| RRID | AB_2714217 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96C03 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMCR7/MID49 antibody 16413-1-AP | Download protocol |
| IHC protocol for SMCR7/MID49 antibody 16413-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division. | ||
Cell Metab Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells. | ||
Nat Cell Biol MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
| ||
Autophagy Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy.
| ||
J Cell Biol The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission. | ||
EMBO J FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.
|
