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SMG5 Polyclonal antibody

SMG5 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12694-1-AP

Print datasheet

Synonyms

KIAA1089, hSMG-5, hSMG 5, EST1-like protein B, EST1B

验证数据展示

  • IHC staining of mouse heart using 12694-1-AP

    IHC staining of mouse heart using 12694-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12694-1-AP (SMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 12694-1-AP

    IF Staining of MCF-7 using 12694-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SMG5 antibody (12694-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • IF Staining of Jurkat using 12694-1-AP

    IF Staining of Jurkat using 12694-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SMG5 antibody (12694-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, Jurkat cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12694-1-AP targets SMG5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SMG5 fusion protein Ag3424 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans)
Synonyms KIAA1089, hSMG-5, hSMG 5, EST1-like protein B, EST1B
Calculated Molecular Weight 1016 aa, 114 kDa
GenBank Accession NumberBC038296
Gene Symbol SMG5
Gene ID (NCBI) 23381
RRIDAB_2270781
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UPR3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SMG5 antibody 12694-1-APDownload protocol
IF protocol for SMG5 antibody 12694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Identification of nonsense-mediated mRNA decay pathway as a critical regulator of p53 isoform β.

Authors - Lauren E Cowen
View Article
humanWB

Sci Rep

Characterization of SMG7 14-3-3-like domain reveals phosphoserine binding-independent regulation of p53 and UPF1.

Authors - Lauren E Cowen
View Article
humanWB

Sci Rep

Critical role of SMG7 in activation of the ATR-CHK1 axis in response to genotoxic stress.

Authors - Kathleen Ho
View Article