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验证数据展示

  • IHC staining of mouse heart using 12694-1-AP

    IHC staining of mouse heart using 12694-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12694-1-AP (SMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 12694-1-AP

    IF Staining of MCF-7 using 12694-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SMG5 antibody (12694-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • IF Staining of Jurkat using 12694-1-AP

    IF Staining of Jurkat using 12694-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using SMG5 antibody (12694-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

SMG5 Polyclonal antibody

SMG5 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 12694-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA and More (1)

别名

KIAA1089, hSMG-5, hSMG 5, EST1-like protein B, EST1B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

12694-1-AP targets SMG5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 SMG5 fusion protein Ag3424 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Smg-5 homolog, nonsense mediated mRNA decay factor (C. elegans)
别名 KIAA1089, hSMG-5, hSMG 5, EST1-like protein B, EST1B
计算分子量 1016 aa, 114 kDa
GenBank蛋白编号BC038296
基因名称 SMG5
Gene ID (NCBI) 23381
RRIDAB_2270781
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPR3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for SMG5 antibody 12694-1-APDownload protocol
IF protocol for SMG5 antibody 12694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Identification of nonsense-mediated mRNA decay pathway as a critical regulator of p53 isoform β.

Authors - Lauren E Cowen
View Article
humanWB

Sci Rep

Characterization of SMG7 14-3-3-like domain reveals phosphoserine binding-independent regulation of p53 and UPF1.

Authors - Lauren E Cowen
View Article
humanWB

Sci Rep

Critical role of SMG7 in activation of the ATR-CHK1 axis in response to genotoxic stress.

Authors - Kathleen Ho
View Article