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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68109-1-Ig (same clone as 68109-1-PBS)

    WB analysis using 68109-1-Ig (same clone as 68109-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68109-1-Ig (SMG6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 68109-1-PBS in a different storage buffer formulation.

  • WB analysis of RAW 264.7 using 68109-1-Ig (same clone as 68109-1-PBS)

    WB analysis of RAW 264.7 using 68109-1-Ig (same clone as 68109-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68109-1-Ig (SMG6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68109-1-PBS in a different storage buffer formulation.

SMG6 Monoclonal antibody, PBS Only

SMG6 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68109-1-PBS

产品说明书

CloneNo. 1D2G2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse

应用

WB, Indirect ELISA

别名

C17orf31, EST1 like protein A, EST1A, Ever shorter telomeres 1A, hSmg5/7a, KIAA0732, SMG 6, Smg 6 homolog, SMG6, Telomerase subunit EST1A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68109-1-PBS targets SMG6 in WB, Indirect ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 Human, mouse
免疫原 SMG6 fusion protein Ag30182 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 Smg-6 homolog, nonsense mediated mRNA decay factor (C. elegans)
别名 C17orf31, EST1 like protein A, EST1A, Ever shorter telomeres 1A, hSmg5/7a, KIAA0732, SMG 6, Smg 6 homolog, SMG6, Telomerase subunit EST1A
计算分子量 160 kDa
观测分子量160 kDa
GenBank蛋白编号NM_017575
基因名称 SMG6
Gene ID (NCBI) 23293
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ86US8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMG6, also known as C17orf31, EST1A, and hEST1A, is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini (PMID: 19179534). It promotes in vitro the ability of TERT to elongate telomeres (PMID: 12676087, 12699629). SMG6 also plays a role in the nonsense-mediated mRNA decay (NMD) pathway, providing the endonuclease activity near the premature translation termination codon that is needed to initiate NMD (PMID: 19060897, 20930030). Alternate splicing results in multiple transcript variants (PMID: 12699629, 14636577).