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SMMHC Monoclonal antibody, PBS Only

SMMHC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

5C6D3

Cat no : 60222-1-PBS

Print datasheet

Synonyms

AAT4, DKFZp686D10126, DKFZp686D19237, FAA4, FLJ35232, KIAA0866, MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain

验证数据展示

  • WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

    WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

    IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

    IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60222-1-Ig (same clone as 60222-1-PBS)

    IF Staining of HeLa using 60222-1-Ig (same clone as 60222-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60222-1-Ig (SMMHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

产品信息

60222-1-PBS targets SMMHC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen SMMHC fusion protein Ag13397 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name myosin, heavy chain 11, smooth muscle
Synonyms AAT4, DKFZp686D10126, DKFZp686D19237, FAA4, FLJ35232, KIAA0866, MYH11, Myosin 11, Myosin heavy chain 11, SMHC, SMMHC, SMM-HC, smooth muscle myosin heavy chain
Calculated Molecular Weight 1938 aa, 224 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC101677
Gene Symbol SMMHC/MYH11
Gene ID (NCBI) 4629
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35749
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.