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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

    WB analysis using 60222-1-Ig (same clone as 60222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60222-1-Ig (SMMHC antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

    IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

    IHC staining of human breast hyperplasia using 60222-1-Ig (same clone as 60222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60222-1-Ig (same clone as 60222-1-PBS)

    IF Staining of HeLa using 60222-1-Ig (same clone as 60222-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60222-1-Ig (SMMHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 60222-1-Ig (same clone as 60222-1-PBS)

    IHC staining of human colon using 60222-1-Ig (same clone as 60222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60222-1-Ig (SMMHC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60222-1-PBS in a different storage buffer formulation.

SMMHC Monoclonal antibody, PBS Only

SMMHC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60222-1-PBS

产品说明书

CloneNo. 5C6D3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA

别名

MYH11, Myosin-11, Myosin heavy chain 11, Myosin 11, KIAA0866

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60222-1-PBS targets SMMHC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 SMMHC fusion protein Ag13397 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 myosin, heavy chain 11, smooth muscle
别名 MYH11, Myosin-11, Myosin heavy chain 11, Myosin 11, KIAA0866
计算分子量 1938 aa, 224 kDa
观测分子量 220 kDa
GenBank蛋白编号BC101677
基因名称 SMMHC/MYH11
Gene ID (NCBI) 4629
RRIDAB_11182594
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP35749
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMMHC (smooth muscle myosin heavy chain; MYH11) is a contractile protein of smooth muscle cells. It is specifically expressed in cells derived from smooth muscle lineages. SMMHC is used as vascular smooth muscle cell (vSMC) contractile marker. It is also an excellent marker for myoepithelial cells, with no or few cross-reaction with myofibroblasts, thus very useful in the evaluation of stromal invasion.