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  • Featured Product
  • KD/KO Validated

SMN Polyclonal antibody

SMN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20451-1-AP

Print datasheet

Synonyms

C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

验证数据展示

  • WB analysis of HeLa using 20451-1-AP

    WB analysis of HeLa using 20451-1-AP

    WB result of SMN antibody (20451-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HeLa cells.

  • WB analysis of HEK-293 using 20451-1-AP

    WB analysis of HEK-293 using 20451-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 20451-1-AP

    IP experiment of HEK-293 using 20451-1-AP

    IP result of anti-SMN (IP:20451-1-AP, 4ug; Detection:20451-1-AP 1:300) with HEK-293 cells lysate 2000 ug.

  • IHC staining of human kidney using 20451-1-AP

    IHC staining of human kidney using 20451-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20451-1-AP (SMN antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 20451-1-AP

    IHC staining of human kidney using 20451-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20451-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 20451-1-AP

    IF Staining of HepG2 using 20451-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (20451-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HepG2 cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20451-1-AP is SMN Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SMN fusion protein Ag14333 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name survival of motor neuron 2, centromeric
Synonyms C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein
Calculated Molecular Weight 282 aa, 30 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000908
Gene Symbol SMN
Gene ID (NCBI) 6607
RRIDAB_10665425
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

实验方案

Product Specific Protocols
WB protocol for SMN antibody 20451-1-APDownload protocol
IHC protocol for SMN antibody 20451-1-APDownload protocol
IF protocol for SMN antibody 20451-1-APDownload protocol
IP protocol for SMN antibody 20451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols