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验证数据展示

  • WB analysis of HeLa using 20451-1-AP

    WB analysis of HeLa using 20451-1-AP

    WB result of SMN antibody (20451-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HeLa cells.

  • WB analysis of HEK-293 using 20451-1-AP

    WB analysis of HEK-293 using 20451-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20451-1-AP (SMN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 20451-1-AP

    IP experiment of HEK-293 using 20451-1-AP

    IP result of anti-SMN (IP:20451-1-AP, 4ug; Detection:20451-1-AP 1:300) with HEK-293 cells lysate 2000 ug.

  • IHC staining of human kidney using 20451-1-AP

    IHC staining of human kidney using 20451-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20451-1-AP (SMN antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 20451-1-AP

    IHC staining of human kidney using 20451-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 20451-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 20451-1-AP

    IF Staining of HepG2 using 20451-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (20451-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

SMN Polyclonal antibody

SMN Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20451-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HepG2 cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20451-1-AP targets SMN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 SMN fusion protein Ag14333 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 survival of motor neuron 2, centromeric
别名 C BCD541, Component of gems 1, Gemin 1, SMN, SMN1, SMN1,SMN, SMN2, SMNC, SMNT, Survival motor neuron protein
计算分子量 282 aa, 30 kDa
观测分子量 38 kDa
GenBank蛋白编号BC000908
基因名称 SMN
Gene ID (NCBI) 6607
RRIDAB_10665425
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16637
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA.

实验方案

Product Specific Protocols
WB protocol for SMN antibody 20451-1-APDownload protocol
IHC protocol for SMN antibody 20451-1-APDownload protocol
IF protocol for SMN antibody 20451-1-APDownload protocol
IP protocol for SMN antibody 20451-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols