验证数据展示
经过测试的应用
Positive IHC detected in | human prostate cancer tissue, human testis tissue, human colon cancer tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 6 publications below |
IF | See 6 publications below |
产品信息
20787-1-AP targets SMO in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IHC, ELISA Application Description |
文献引用应用 | IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, chicken |
免疫原 | SMO fusion protein Ag14322 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | smoothened homolog (Drosophila) |
别名 | Smoothened homolog, SMOH, Protein smoothened, Protein Gx, Gx |
计算分子量 | 787 aa, 86 kDa |
GenBank蛋白编号 | BC009989 |
基因名称 | SMO |
Gene ID (NCBI) | 6608 |
RRID | AB_2878740 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q99835 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays a central role in animal development and stem-cell function. Defects in Hh signaling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.
实验方案
Product Specific Protocols | |
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IHC protocol for SMO antibody 20787-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cancer Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. | ||
Sci Adv Primary cilium remodeling mediates a cell signaling switch in differentiating neurons. | ||
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. | ||
J Cell Biol Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway. | ||
Acta Neuropathol Commun Meningioma cells express primary cilia but do not transduce ciliary Hedgehog signals. | ||
Cell Death Discov N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness |