验证数据展示
经过测试的应用
| Positive IHC detected in | human prostate cancer tissue, human testis tissue, human colon cancer tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SMO Ready-To-Use IHC Kit. SMO primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 6 publications below |
| IF | See 6 publications below |
产品信息
20787-1-AP targets SMO in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, ELISA Application Description |
| Cited Applications | IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Immunogen | SMO fusion protein Ag14322 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | smoothened homolog (Drosophila) |
| Synonyms | Smoothened homolog, SMOH, Protein smoothened, Protein Gx, Gx |
| Calculated Molecular Weight | 787 aa, 86 kDa |
| GenBank Accession Number | BC009989 |
| Gene Symbol | SMO |
| Gene ID (NCBI) | 6608 |
| RRID | AB_2878740 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99835 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays a central role in animal development and stem-cell function. Defects in Hh signaling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SMO antibody 20787-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. | ||
Sci Adv Primary cilium remodeling mediates a cell signaling switch in differentiating neurons. | ||
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. | ||
J Cell Biol Sterol and oxysterol synthases near the ciliary base activate the Hedgehog pathway. | ||
Acta Neuropathol Commun Meningioma cells express primary cilia but do not transduce ciliary Hedgehog signals. | ||
Cell Death Discov N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness |
