SMO Monoclonal antibody

SMO Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 66851-1-Ig

产品说明书

CloneNo. 2B10A11

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Canine, Mouse, Rat and More (3)

应用

WB, IF, IHC, ELISA

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy SMO Ready-To-Use IHC Kit. SMO primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66851-1-Ig targets SMO in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Canine, Mouse, Rat samples.

经测试应用 WB, IF, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 Human, Canine, Mouse, Rat
文献引用反应性human, mouse, rat
免疫原 SMO fusion protein Ag25861 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 smoothened homolog (Drosophila)
别名 Gx, Protein Gx, SMO, SMOH, Smoothened homolog
计算分子量 787 aa, 86 kDa
观测分子量86 kDa
GenBank蛋白编号BC009989
基因名称 SMO
Gene ID (NCBI) 6608
RRIDAB_2882191
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99835
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

实验方案

Product Specific Protocols
WB protocol for SMO antibody 66851-1-IgDownload protocol
IHC protocol for SMO antibody 66851-1-IgDownload protocol
IF protocol for SMO antibody 66851-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Int J Biol Sci

Drug Repurposing Flubendazole to Suppress Tumorigenicity via PCSK9-dependent Inhibition and Potentiate Lenvatinib Therapy for Hepatocellular Carcinoma

Authors - Wenjiao Jin
human,mouseWB

iScience

Mutational burden of XPNPEP3 leads to defects in mitochondrial complex I and cilia in NPHPL1

Authors - Lingxiao Tong
humanWB

Front Cell Dev Biol

Microtubule Stabilization Enhances the Chondrogenesis of Synovial Mesenchymal Stem Cells.

Authors - Jiawei Li
ratWB

Oxid Med Cell Longev

Mitochondrial Protection and Against Glutamate Neurotoxicity via Shh/Ptch1 Signaling Pathway to Ameliorate Cognitive Dysfunction by Kaixin San in Multi-Infarct Dementia Rats

Authors - Xiaoqiong Li
humanWB

Cancer Sci

Transcription factor POU4F2 promotes colorectal cancer cell migration and invasion through hedgehog-mediated epithelial-mesenchymal transition.

Authors - Kaibo Guo
humanWB

FASEB J

Inhibition of CK2α accelerates skin wound healing by promoting endothelial cell proliferation through the Hedgehog signaling pathway

Authors - Junjie Zhu
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