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  • Featured Product
  • KD/KO Validated

SMOX Polyclonal antibody

SMOX Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15052-1-AP

Print datasheet

Synonyms

C20orf16, PAO, PAO 1, PAOh1, Polyamine oxidase 1, SMO, SMOX, spermine oxidase

验证数据展示

  • WB analysis of PC-13 using 15052-1-AP

    WB analysis of PC-13 using 15052-1-AP

    PC-13 cells were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 15052-1-AP

    WB analysis using 15052-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15052-1-AP (SMOX antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 15052-1-AP

    IHC staining of human prostate cancer using 15052-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 15052-1-AP

    IHC staining of human prostate cancer using 15052-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 15052-1-AP

    IHC staining of human stomach cancer using 15052-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 15052-1-AP

    IHC staining of human stomach cancer using 15052-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 15052-1-AP (SMOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, PC-13 cells, MCF-7 cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human stomach cancer tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15052-1-AP targets SMOX in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen SMOX fusion protein Ag7035 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name spermine oxidase
Synonyms C20orf16, PAO, PAO 1, PAOh1, Polyamine oxidase 1, SMO, SMOX, spermine oxidase
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 56-65 kDa
GenBank Accession NumberBC000669
Gene Symbol SMOX
Gene ID (NCBI) 54498
RRIDAB_2239689
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWM0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMOX(Spermine oxidase) is also named as SMO, PAO-1 and PAOh1. It belongs to the class of polyamine oxidases (PAOs).The flavoprotein SMO, which specifically oxidizes Spm, is one of the key regulatory enzymes involved in polyamine homeostasis in animal cells (PMID:14764092). It has 6 isoforms produced by alternative splicing with the MW of 61 kDa, 56 kDa, 21 kDa, 60 kDa, 43 kDa and 65 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for SMOX antibody 15052-1-APDownload protocol
IHC protocol for SMOX antibody 15052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Clin Invest

Inhibition of the aryl hydrocarbon receptor/polyamine biosynthesis axis suppresses multiple myeloma.

Authors - Anna Bianchi-Smiraglia
View Article
mouseWB

J Clin Invest

Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology.

Authors - Leslie A Sandusky-Beltran
View Article
mouseWB

EBioMedicine

Silencing of circular RNA HIPK2 in neural stem cells enhances functional recovery following ischaemic stroke.

Authors - Guangtian Wang
View Article
humanWB

J Invest Dermatol

The Polyamine Putrescine Promotes Human Epidermal Melanogenesis.

Authors - Aishwarya Sridharan
View Article

Free Radic Biol Med

Adaptive responses of heart and skeletal muscle to spermine oxidase overexpression: Evaluation of a new transgenic mouse model.

Authors - Roberta Ceci
View Article
humanWB,IHC

Biomedicines

Expression of Spermine Oxidase Is Associated with Colorectal Carcinogenesis and Prognosis of Patients.

Authors - Sooyoun Kim
View Article