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SMPD1,ASM Polyclonal antibody

SMPD1,ASM Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30197-1-AP

Print datasheet

Synonyms

SMPD1, Sphingomyelin phosphodiesterase, EC:3.1.4.12, aSMase, ASM

验证数据展示

  • WB analysis of human placenta using 30197-1-AP

    WB analysis of human placenta using 30197-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30197-1-AP (SMPD1,ASM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 30197-1-AP

    IF Staining of NIH/3T3 using 30197-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using SMPD1,ASM antibody (30197-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30197-1-AP targets SMPD1,ASM in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SMPD1,ASM fusion protein Ag32479 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sphingomyelin phosphodiesterase 1, acid lysosomal
Synonyms SMPD1, Sphingomyelin phosphodiesterase, EC:3.1.4.12, aSMase, ASM
Calculated Molecular Weight 70 kDa
Observed Molecular Weight68-70 kDa
GenBank Accession NumberBC041164
Gene Symbol SMPD1
Gene ID (NCBI) 6609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17405
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMPD1(Sphingomyelin phosphodiesterase) is also named as ASM and belongs to the acid sphingomyelinase family. It is an important enzyme in sphingolipid metabolism and plays key roles in apoptosis, immunity, development, and cancer. The deficient activity of this enzyme results in Types A and B Niemann-Pick disease (NPD). This protein has 4 isoforms produced by alternative splicing. SMPD1 can produce a 60 kDa peptide with its signal peptide missing. As a catalytically inactive 75kDa precursor, after signal peptide cleavage, it was processed into smaller non-glycosylated and rapidly degraded 57kDa form in ER-Golgi complex. And 70kDa mature form with major catalytic activity located in lysosomes, which is further processed into 52kDa form (PMID:25853898, PMID:26499107).

实验方案

Product Specific Protocols
WB protocol for SMPD1,ASM antibody 30197-1-APDownload protocol
IF protocol for SMPD1,ASM antibody 30197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols