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SMPD2 Polyclonal antibody

SMPD2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 15239-1-AP

Print datasheet

Synonyms

Lyso PAF PLC, N SMase, Neutral sphingomyelinase, NSMASE, NSMASE1, SMPD2

验证数据展示

  • WB analysis of mouse liver using 15239-1-AP

    WB analysis of mouse liver using 15239-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15239-1-AP (SMPD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15239-1-AP targets SMPD2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SMPD2 fusion protein Ag7367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase)
Synonyms Lyso PAF PLC, N SMase, Neutral sphingomyelinase, NSMASE, NSMASE1, SMPD2
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48 kDa
GenBank Accession NumberBC000038
Gene Symbol SMPD2
Gene ID (NCBI) 6610
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60906
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMPD2, Sphingomyelin phosphodiesterase 2, is also named as Lyso-platelet-activating factor-phospholipase C and neutral sphingomyelinase (nSMase). It is involved in the sphingolipid metabolism pathway, which can catalyze the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Smpd2/Smpd3 double-mutant mice were found to have severe retardation of late embryonic and postnatal growth and delayed puberty, belonging to hypothalamus-induced combined pituitary hormone deficiency (PubMed: 15764706).

实验方案

Product Specific Protocols
WB protocol for SMPD2 antibody 15239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols