验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells |
| Positive IHC detected in | human hysteromyoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy Smoothelin/SMTN Ready-To-Use IHC Kit. Smoothelin/SMTN primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IHC | See 2 publications below |
产品信息
23567-1-AP targets SMTN in WB, IHC, ELISA applications and shows reactivity with mouse, human, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | mouse, human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SMTN fusion protein Ag19983 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | smoothelin |
| 别名 | smoothelin, SMSMO, SMTN |
| 计算分子量 | 940 aa, 102 kDa |
| 观测分子量 | 117 kDa |
| GenBank蛋白编号 | BC034237 |
| 基因名称 | SMTN |
| Gene ID (NCBI) | 6525 |
| RRID | AB_2879298 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P53814 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMTN, also named as Smoothelin, is a 917 amino acid protein, which contains 1 CH (calponin-homology) domain and belongs to the smoothelin family. SMTN as structural protein of the cytoskeleton involve in muscle organ development and smooth muscle contraction.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMTN antibody 23567-1-AP | Download protocol |
| IHC protocol for SMTN antibody 23567-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
bioRxiv Coordination between cytoskeletal organization, cell contraction and extracellular matrix development, is depended on LOX for aneurysm prevention | ||
FEBS J Coordination among cytoskeletal organization, cell contraction, and extracellular matrix development is dependent on LOX for aneurysm prevention | ||
Int J Nanomedicine Glycosylation-Engineered Platelet Membrane-Coated Interleukin 10 Nanoparticles for Targeted Inhibition of Vascular Restenosis | ||
J Mol Med (Berl) Vav2 promotes ductus arteriosus anatomic closure via the remodeling of smooth muscle cells by Rac1 activation |
