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SMTNL2 Polyclonal antibody

SMTNL2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14782-1-AP

Print datasheet

Synonyms

Smoothelin-like protein 2, Smoothelin like protein 2, smoothelin like 2

验证数据展示

  • WB analysis of COLO 320 using 14782-1-AP

    WB analysis of COLO 320 using 14782-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14782-1-AP (SMTNL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 14782-1-AP

    IP experiment of COLO 320 using 14782-1-AP

    IP result of anti-SMTNL2 (IP:14782-1-AP, 4ug; Detection:14782-1-AP 1:500) with COLO 320 cells lysate 4000ug.

  • IF Staining of MCF-7 using 14782-1-AP

    IF Staining of MCF-7 using 14782-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 14782-1-AP (SMTNL2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCOLO 320 cells, MCF-7 cells, mouse small intestine tissue, SGC-7901 cells
Positive IP detected inCOLO 320 cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14782-1-AP targets SMTNL2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SMTNL2 fusion protein Ag6395 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name smoothelin-like 2
Synonyms Smoothelin-like protein 2, Smoothelin like protein 2, smoothelin like 2
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 68-70 kDa
GenBank Accession NumberBC112177
Gene Symbol SMTNL2
Gene ID (NCBI) 342527
RRIDAB_2270842
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2TAL5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SMTNL2 antibody 14782-1-APDownload protocol
IF protocol for SMTNL2 antibody 14782-1-APDownload protocol
IP protocol for SMTNL2 antibody 14782-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols