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验证数据展示

  • IF Staining of MCF-7 using 66330-1-Ig (same clone as 66330-1-PBS)

    IF Staining of MCF-7 using 66330-1-Ig (same clone as 66330-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using SMYD3 antibody (66330-1-Ig, Clone: 1B5C10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 66330-1-Ig (same clone as 66330-1-PBS)

    WB analysis of A431 using 66330-1-Ig (same clone as 66330-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66330-1-Ig (SMYD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66330-1-Ig (same clone as 66330-1-PBS)

    IHC staining of human pancreas cancer using 66330-1-Ig (same clone as 66330-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66330-1-Ig (SMYD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66330-1-PBS in a different storage buffer formulation.

SMYD3 Monoclonal antibody, PBS Only

SMYD3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66330-1-PBS

产品说明书

CloneNo. 1B5C10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

SMYD3, ZMYND1, ZNFN3A1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66330-1-PBS targets SMYD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 SMYD3 fusion protein Ag2624 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SET and MYND domain containing 3
别名 SMYD3, ZMYND1, ZNFN3A1
计算分子量428aa,49 kDa; 369aa,42 kDa
观测分子量 49 kDa
GenBank蛋白编号BC031010
基因名称 SMYD3
Gene ID (NCBI) 64754
RRIDAB_2881711
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9H7B4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer(PMID:16155568). Mainly cytoplasmic when cells are arrested at G0/G1, accumulates in the nucleus at S phase and G2/M(Uniprot).