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验证数据展示

  • WB analysis of HeLa using 82214-2-RR

    WB analysis of HeLa using 82214-2-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 82214-2-RR (SNAI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82214-2-RR

    Affinity and Kinetic Characterization of 82214-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82214-2-RR against Human SNAI1 were performed. The affinity constant is below 1 pM.

SNAI1 Recombinant antibody

SNAI1 Recombinant Antibody for WB, ELISA
Cat No. 82214-2-RR

产品说明书

CloneNo. 230035C9

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

dJ710H13.1, Protein sna, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI1, snail homolog 1 (Drosophila), SNAIL1, Zinc finger protein SNAI1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82214-2-RR targets SNAI1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 SNAI1 fusion protein Ag3723 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 snail homolog 1 (Drosophila)
别名 dJ710H13.1, Protein sna, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI1, snail homolog 1 (Drosophila), SNAIL1, Zinc finger protein SNAI1
计算分子量 264 aa, 29 kDa
观测分子量35 kDa
GenBank蛋白编号BC012910
基因名称 SNAI1
Gene ID (NCBI) 6615
RRIDAB_3670519
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO95863
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNAI1, a member of SNAI1 family of protein, participates in the epithelial to mesenchymal transition(EMT) and formation and maintenance of embryonic mesoderm. The snail family share a common structural, that a highly conserved C-terminal region containing a zinc finger transcription factor. SNAI1 interacts with other corepressor, such as Ajuba, PRMT5 and SIN3a or HDAC1 and 2, to repress the target gene. As the phosphorylation modification of SNAI1 protein, the range of molecular weight of SNAI1 is about 25-30 kDa (PMID: 22276203 ). Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.

实验方案

Product Specific Protocols
WB protocol for SNAI1 antibody 82214-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols