SNAI2/SLUG Polyclonal antibody

SNAI2/SLUG Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 12129-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, FC (Intra), ELISA and More (3)

SNAI2, WS2D, SLUGH1, SLUGH, SLUG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse testis tissue, mouse brain tissue, rat heart tissue, mouse spleen tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12129-1-AP targets SNAI2/SLUG in WB, IHC, IF, FC (Intra), CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF, CoIP, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, zebrafish
免疫原 SNAI2/SLUG fusion protein Ag2773 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 snail homolog 2 (Drosophila)
别名 SNAI2, WS2D, SLUGH1, SLUGH, SLUG
计算分子量 268 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC015895
基因名称 SLUG
Gene ID (NCBI) 6591
RRIDAB_2191889
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43623
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.

实验方案

Product Specific Protocols
WB protocol for SNAI2/SLUG antibody 12129-1-APDownload protocol
IHC protocol for SNAI2/SLUG antibody 12129-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK

Authors - Li Cui
humanWB

J Exp Clin Cancer Res

Lnc AC016727.1/BACH1/HIF-1 α signal loop promotes the progression of non-small cell lung cancer

Authors - Li Zhang
humanWB

Cell Death Dis

UBE2E2 enhances Snail-mediated epithelial-mesenchymal transition and Nrf2-mediated antioxidant activity in ovarian cancer

Authors - Xiaoling Hong
humanWB

Mol Ther

IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.

Authors - Hailong Ruan
mouseIF, WB

Theranostics

Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development.

Authors - Shuyu Guo
humanWB

Cell Death Dis

ZC3H15 promotes glioblastoma progression through regulating EGFR stability.

Authors - Jianbing Hou
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