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验证数据展示

  • WB analysis of mouse brain using 17818-1-AP

    WB analysis of mouse brain using 17818-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17818-1-AP (SNCAIP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 17818-1-AP

    IP experiment of mouse brain using 17818-1-AP

    IP result of anti-SNCAIP (IP:17818-1-AP, 4ug; Detection:17818-1-AP 1:300) with mouse brain tissue lysate 3600ug.

  • IHC staining of human kidney using 17818-1-AP

    IHC staining of human kidney using 17818-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17818-1-AP (SNCAIP Antibody) at dilution of 1:50 (under 40x lens).

SNCAIP Polyclonal antibody

SNCAIP Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17818-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

SNCAIP, Sph1, Synphilin 1, SYPH1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, fetal human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17818-1-AP targets SNCAIP in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原 SNCAIP fusion protein Ag12147 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synuclein, alpha interacting protein
别名 SNCAIP, Sph1, Synphilin 1, SYPH1
计算分子量603aa,67 kDa; 1016aa,111 kDa
观测分子量 100 kDa, 75 kDa
GenBank蛋白编号BC094759
基因名称 SNCAIP
Gene ID (NCBI) 9627
RRIDAB_2878444
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6H5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNCAIP, also named as Synphilin-1 or Alpha-synuclein-interacting protein, is a 919 amino acid protein, which contains six ANK repeats. SNCAIP is detected in brain and is widely expressed, with highest levels in brain, heart and placenta. SNCAIP localizes in the cytoplasm and inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. SNCAIP has an association with Parkinson disease.

实验方案

Product Specific Protocols
WB protocol for SNCAIP antibody 17818-1-APDownload protocol
IHC protocol for SNCAIP antibody 17818-1-APDownload protocol
IP protocol for SNCAIP antibody 17818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Neurosci Lett

Synphilin-1 has neuroprotective effects on MPP+-induced Parkinson's disease model cells by inhibiting ROS production and apoptosis.

Authors - Takeo Shishido
View Article