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SNCAIP Polyclonal antibody

SNCAIP Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17818-1-AP

Print datasheet

Synonyms

SNCAIP, Sph1, Synphilin 1, SYPH1

验证数据展示

  • WB analysis of mouse brain using 17818-1-AP

    WB analysis of mouse brain using 17818-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17818-1-AP (SNCAIP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 17818-1-AP

    IP experiment of mouse brain using 17818-1-AP

    IP result of anti-SNCAIP (IP:17818-1-AP, 4ug; Detection:17818-1-AP 1:300) with mouse brain tissue lysate 3600ug.

  • IHC staining of human kidney using 17818-1-AP

    IHC staining of human kidney using 17818-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17818-1-AP (SNCAIP Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue, fetal human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 17818-1-AP is SNCAIP Fusion Protein expressed in E. coli.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SNCAIP fusion protein Ag12147 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synuclein, alpha interacting protein
Synonyms SNCAIP, Sph1, Synphilin 1, SYPH1
Calculated Molecular Weight603aa,67 kDa; 1016aa,111 kDa
Observed Molecular Weight 100 kDa, 75 kDa
GenBank Accession NumberBC094759
Gene Symbol SNCAIP
Gene ID (NCBI) 9627
RRIDAB_2878444
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6H5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNCAIP, also named as Synphilin-1 or Alpha-synuclein-interacting protein, is a 919 amino acid protein, which contains six ANK repeats. SNCAIP is detected in brain and is widely expressed, with highest levels in brain, heart and placenta. SNCAIP localizes in the cytoplasm and inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. SNCAIP has an association with Parkinson disease.

实验方案

Product Specific Protocols
WB protocol for SNCAIP antibody 17818-1-APDownload protocol
IHC protocol for SNCAIP antibody 17818-1-APDownload protocol
IP protocol for SNCAIP antibody 17818-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Neurosci Lett

Synphilin-1 has neuroprotective effects on MPP+-induced Parkinson's disease model cells by inhibiting ROS production and apoptosis.

Authors - Takeo Shishido
View Article