验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, fetal human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
17818-1-AP targets SNCAIP in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | SNCAIP fusion protein Ag12147 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | synuclein, alpha interacting protein |
| Synonyms | SNCAIP, Sph1, Synphilin 1, SYPH1 |
| Calculated Molecular Weight | 603aa,67 kDa; 1016aa,111 kDa |
| Observed Molecular Weight | 100 kDa, 75 kDa |
| GenBank Accession Number | BC094759 |
| Gene Symbol | SNCAIP |
| Gene ID (NCBI) | 9627 |
| RRID | AB_2878444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6H5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SNCAIP, also named as Synphilin-1 or Alpha-synuclein-interacting protein, is a 919 amino acid protein, which contains six ANK repeats. SNCAIP is detected in brain and is widely expressed, with highest levels in brain, heart and placenta. SNCAIP localizes in the cytoplasm and inhibits the ubiquitin ligase activity of SIAH1 and inhibits proteasomal degradation of target proteins. SNCAIP has an association with Parkinson disease.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNCAIP antibody 17818-1-AP | Download protocol |
| IHC protocol for SNCAIP antibody 17818-1-AP | Download protocol |
| IP protocol for SNCAIP antibody 17818-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
