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SNCB Polyclonal antibody

SNCB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10498-1-AP

Print datasheet

Synonyms

Beta-synuclein, Beta synuclein

验证数据展示

  • WB analysis of mouse brain using 10498-1-AP

    WB analysis of mouse brain using 10498-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10498-1-AP (SNCB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 10498-1-AP

    IHC staining of human breast cancer using 10498-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10498-1-AP (SNCB antibody) at dilution of 1:10 (under 10x lens).

  • IF Staining of HepG2 using 10498-1-AP

    IF Staining of HepG2 using 10498-1-AP

    Immunofluorescent analysis of HepG2 cells, using SNCB antibody 10498-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of mouse brain using 10498-1-AP

    IP experiment of mouse brain using 10498-1-AP

    IP result of anti-SNCB (IP:10498-1-AP, 4ug; Detection:10498-1-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10498-1-AP targets SNCB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SNCB fusion protein Ag0772 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synuclein, beta
Synonyms Beta-synuclein, Beta synuclein
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-18 kDa
GenBank Accession NumberBC002902
Gene Symbol SNCB
Gene ID (NCBI) 6620
RRIDAB_2270787
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16143
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SNCB antibody 10498-1-APDownload protocol
IHC protocol for SNCB antibody 10498-1-APDownload protocol
IF protocol for SNCB antibody 10498-1-APDownload protocol
IP protocol for SNCB antibody 10498-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols