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验证数据展示

  • WB analysis using 16398-1-AP

    WB analysis using 16398-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16398-1-AP (SNCG antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 16398-1-AP

    IHC staining of mouse brain using 16398-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16398-1-AP (SNCG antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 16398-1-AP

    IHC staining of mouse brain using 16398-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16398-1-AP (SNCG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SNCG Polyclonal antibody

SNCG Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16398-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Gamma synuclein, Persyn, PRSN, SNCG, SR, Synoretin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHT-29 cells, HeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16398-1-AP targets SNCG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SNCG fusion protein Ag9493 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synuclein, gamma (breast cancer-specific protein 1)
Synonyms Gamma synuclein, Persyn, PRSN, SNCG, SR, Synoretin
Calculated Molecular Weight 127 aa, 13 kDa
Observed Molecular Weight13 kDa
GenBank Accession NumberBC014098
Gene Symbol SNCG
Gene ID (NCBI) 6623
RRIDAB_3085489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO76070
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Gamma synuclein, also known as SNCG, BCSG1, belongs to the synuclein family. SNCG is a synuclein protein found primarily in the peripheral nervous system and retina(PMID 11806835). SNCG is the least conserved of the synuclein proteins and is detected in the brain, ovarian tumors, and the olfactory epithelium(PMID 9750188). SNCG is expressed in high abundance in breast cancer but scarcely at all in a normal breast and is considered a breast cancer marker(PMID: 10813729).

实验方案

Product Specific Protocols
WB protocol for SNCG antibody 16398-1-APDownload protocol
IHC protocol for SNCG antibody 16398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols