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  • Featured Product
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RCC1 Polyclonal antibody

RCC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10697-1-AP

Print datasheet

Synonyms

RCC1, SNHG3 RCC1, SNHG3-RCC1

验证数据展示

  • WB analysis of HEK-293 using 10697-1-AP

    WB analysis of HEK-293 using 10697-1-AP

    WB result of RCC1 antibody (10697-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCC1 transfected HEK-293 cells.

  • WB analysis using 10697-1-AP

    WB analysis using 10697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 10697-1-AP

    IP experiment of HeLa using 10697-1-AP

    IP result of anti-RCC1 (IP:10697-1-AP, 4ug; Detection:10697-1-AP 1:500) with HeLa cells lysate 1600 ug.

  • IHC staining of human ovary tumor using 10697-1-AP

    IHC staining of human ovary tumor using 10697-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 10697-1-AP

    IHC staining of human ovary tumor using 10697-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, mouse testis tissue, Raji cells, SGC-7901 cells, HeLa cells, HEK-293 cells, HEK-293T cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10697-1-AP targets RCC1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RCC1 fusion protein Ag1042 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SNHG3-RCC1 readthrough transcript
Synonyms RCC1, SNHG3 RCC1, SNHG3-RCC1
Calculated Molecular Weight 45 kDa, 48 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC007300
Gene Symbol RCC1
Gene ID (NCBI) 751867
RRIDAB_513962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.

实验方案

Product Specific Protocols
WB protocol for RCC1 antibody 10697-1-APDownload protocol
IHC protocol for RCC1 antibody 10697-1-APDownload protocol
IP protocol for RCC1 antibody 10697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols