• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HEK-293 using 10697-1-AP

    WB analysis of HEK-293 using 10697-1-AP

    WB result of RCC1 antibody (10697-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RCC1 transfected HEK-293 cells.

  • WB analysis using 10697-1-AP

    WB analysis using 10697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10697-1-AP (RCC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 10697-1-AP

    IP experiment of HeLa using 10697-1-AP

    IP result of anti-RCC1 (IP:10697-1-AP, 4ug; Detection:10697-1-AP 1:500) with HeLa cells lysate 1600 ug.

  • IHC staining of human ovary tumor using 10697-1-AP

    IHC staining of human ovary tumor using 10697-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RCC1 antibody included
  • IHC staining of human ovary tumor using 10697-1-AP

    IHC staining of human ovary tumor using 10697-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10697-1-AP (RCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RCC1 antibody included
  • Featured Product
  • KD/KO验证

RCC1 Polyclonal antibody

RCC1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 10697-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

RCC1, SNHG3 RCC1, SNHG3-RCC1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, mouse testis tissue, Raji cells, SGC-7901 cells, HeLa cells, HEK-293 cells, HEK-293T cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy RCC1 Ready-To-Use IHC Kit. RCC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10697-1-AP targets RCC1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 RCC1 fusion protein Ag1042 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulator of chromosome condensation 1
别名 RCC1, SNHG3 RCC1, SNHG3-RCC1
计算分子量 45 kDa, 48 kDa
观测分子量 68 kDa
GenBank蛋白编号BC007300
基因名称 RCC1
Gene ID (NCBI) 1104
RRIDAB_513962
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18754
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.

实验方案

Product Specific Protocols
WB protocol for RCC1 antibody 10697-1-APDownload protocol
IHC protocol for RCC1 antibody 10697-1-APDownload protocol
IP protocol for RCC1 antibody 10697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols