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验证数据展示

  • WB analysis using 82962-1-RR

    WB analysis using 82962-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82962-1-RR (SNIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82962-1-RR

    IF Staining of HeLa using 82962-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SNIP1 antibody (82962-1-RR, Clone: 230287D11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • FC experiment of U2OS using 82962-1-RR

    FC experiment of U2OS using 82962-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SNIP1 Recombinant antibody (82962-1-RR, Clone:230287D11) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SNIP1 Recombinant antibody

SNIP1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 82962-1-RR

产品说明书

CloneNo. 230287D11

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

dJ423B22.2, FLJ12553, RP3 423B22.3, SNIP1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, HT-29 cells, U2OS cells, BxPC-3 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells
Positive FC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy SNIP1 Ready-To-Use IHC Kit. SNIP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82962-1-RR targets SNIP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 Human
免疫原 SNIP1 fusion protein Ag33543 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Smad nuclear interacting protein 1
别名 dJ423B22.2, FLJ12553, RP3 423B22.3, SNIP1
计算分子量 46 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC027040
基因名称 SNIP1
Gene ID (NCBI) 79753
RRIDAB_3670708
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ8TAD8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation.

实验方案

Product Specific Protocols
WB protocol for SNIP1 antibody 82962-1-RRDownload protocol
IF protocol for SNIP1 antibody 82962-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols