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验证数据展示

  • IHC staining of mouse cerebellum using 19890-1-AP

    IHC staining of mouse cerebellum using 19890-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19890-1-AP (SNRNP25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 19890-1-AP

    IHC staining of mouse cerebellum using 19890-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 19890-1-AP (SNRNP25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SNRNP25 Polyclonal antibody

SNRNP25 Polyclonal Antibody for IHC, ELISA
Cat No. 19890-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

C16orf33, Minus 99 protein, SNRNP25, U11/U12 25K, U11/U12 snRNP 25 kDa protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19890-1-AP targets SNRNP25 in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SNRNP25 fusion protein Ag13724 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small nuclear ribonucleoprotein 25kDa (U11/U12)
Synonyms C16orf33, Minus 99 protein, SNRNP25, U11/U12 25K, U11/U12 snRNP 25 kDa protein
Calculated Molecular Weight 132 aa, 15 kDa
GenBank Accession NumberBC001381
Gene Symbol SNRNP25
Gene ID (NCBI) 79622
RRIDAB_3085590
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV90
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SNRNP25, small nuclear ribonucleoprotein U11/U12 subunit 25, encodes a 25K protein that is a component of the U12-type spliceosome. Two types of spliceosomes catalyze splicing of pre-mRNAs. The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns. The U12-type spliceosome consists of several small nuclear RNAs and associated proteins. LRP1-SNRNP25 fusion gene promotes osteosarcoma cell invasion and migration (PMID: 26738504, PMID: 25300797).

实验方案

Product Specific Protocols
IHC protocol for SNRNP25 antibody 19890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols