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  • KD/KO Validated

SNRPA Polyclonal antibody

SNRPA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (3)

Conjugate

Unconjugated

Cat no : 10212-1-AP

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Synonyms

U1-A, U1A, U1 snRNP A, U1 A



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, A431 cells, HepG2 cells, mouse liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human cervical cancer tissue, human colon tissue, human liver tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10212-1-AP targets SNRPA in WB, IHC, IF/ICC, IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, ChIP, RIP
Tested Reactivity human, mouse
Cited Reactivityhuman, canine
Immunogen SNRPA fusion protein Ag0283 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small nuclear ribonucleoprotein polypeptide A
Synonyms U1-A, U1A, U1 snRNP A, U1 A
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC000405
Gene Symbol SNRPA
Gene ID (NCBI) 6626
RRIDAB_2239723
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09012
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Heterogeneous nuclear ribonucleoproteins (hnRNPs) constitute a set of polypeptides that contribute to mRNA transcription, premRNA processing as well as mature mRNA transport to the cytoplasm and translation. They also bind heterogeneous nuclear RNA (hnRNA), which are the transcripts produced by RNA polymerase II. hnRNP A1 functions in the packaging of nascent RNA polymerase II transcripts and participates in a variety of nuclear and cytoplasmic processes that modulate gene expression. The RNA binding characteristics of hnRNP A1 suggest that it can modulate the expression of specific genes.

实验方案

Product Specific Protocols
WB protocol for SNRPA antibody 10212-1-APDownload protocol
IHC protocol for SNRPA antibody 10212-1-APDownload protocol
IF protocol for SNRPA antibody 10212-1-APDownload protocol
IP protocol for SNRPA antibody 10212-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,ChIP

Nucleic Acids Res

MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation

Authors - Bangming Jin
humanWB,IHC,IF

Cell Prolif

SNRPA enhances tumour cell growth in gastric cancer through modulating NGF expression.

Authors - Ning Dou
  • KD Validated
canineWB

Biochem Biophys Res Commun

Basolateral sorting of the Mg²⁺ transporter CNNM4 requires interaction with AP-1A and AP-1B.

Authors - Yusuke Hirata
  • KD Validated
RIP

Cell Discov

Postsynaptic lncRNA Sera/Pkm2 pathway orchestrates the transition from social competition to rank by remodeling the neural ensemble in mPFC

Authors - Ling-Shuang Zhu
humanRIP

Mol Cell

Transcription elongation defects link oncogenic SF3B1 mutations to targetable alterations in chromatin landscape

Authors - Prajwal C Boddu
humanWB

Front Oncol

Designer Exosomes for Targeted Delivery of a Novel Therapeutic Cargo to Enhance Sorafenib-Mediated Ferroptosis in Hepatocellular Carcinoma

Authors - Xiaoju Li