验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human brain tissue, human kidney tissue, human liver tissue, mouse liver tissue, mouse lung tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13512-1-AP targets SNRPB2 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SNRPB2 fusion protein Ag4436 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | small nuclear ribonucleoprotein polypeptide B'' |
| 别名 | U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B'' |
| 计算分子量 | 225 aa, 26, 5 kDa |
| 观测分子量 | 25-31 kDa |
| GenBank蛋白编号 | BC036737 |
| 基因名称 | SNRPB2 |
| Gene ID (NCBI) | 6629 |
| RRID | AB_2302390 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P08579 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNRPB2 antibody 13512-1-AP | Download protocol |
| IHC protocol for SNRPB2 antibody 13512-1-AP | Download protocol |
| IF protocol for SNRPB2 antibody 13512-1-AP | Download protocol |
| IP protocol for SNRPB2 antibody 13512-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Transl Med Comment on "Drug screening for ALS using patient-specific induced pluripotent stem cells". | ||
Sci Transl Med Drug screening for ALS using patient-specific induced pluripotent stem cells. | ||
Nucleic Acids Res Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways. | ||
Cell Chem Biol Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer. | ||
STAR Protoc Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis. |
