SNRPB2 Polyclonal antibody

SNRPB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 13512-1-AP

Print datasheet

Synonyms

U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B''



经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, human kidney tissue, human liver tissue, mouse liver tissue, mouse lung tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13512-1-AP targets SNRPB2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IP, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SNRPB2 fusion protein Ag4436 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small nuclear ribonucleoprotein polypeptide B''
Synonyms U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B''
Calculated Molecular Weight 225 aa, 26, 5 kDa
Observed Molecular Weight25-31 kDa
GenBank Accession NumberBC036737
Gene Symbol SNRPB2
Gene ID (NCBI) 6629
RRIDAB_2302390
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08579
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.

实验方案

Product Specific Protocols
WB protocol for SNRPB2 antibody 13512-1-APDownload protocol
IHC protocol for SNRPB2 antibody 13512-1-APDownload protocol
IF protocol for SNRPB2 antibody 13512-1-APDownload protocol
IP protocol for SNRPB2 antibody 13512-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

CPEB1 coordinates alternative 3'-UTR formation with translational regulation.

Authors - Felice-Alessio Bava
humanWB,IHC

Sci Transl Med

Comment on "Drug screening for ALS using patient-specific induced pluripotent stem cells".

Authors - Bilada Bilican
humanIF

Sci Transl Med

Drug screening for ALS using patient-specific induced pluripotent stem cells.

Authors - Naohiro Egawa
humanWB

Nucleic Acids Res

Stoichiometries of U2AF35, U2AF65 and U2 snRNP reveal new early spliceosome assembly pathways.

Authors - Li Chen
humanWB

Cell Chem Biol

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Authors - Li Wang
humanWB

STAR Protoc

Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis.

Authors - Li Wang