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SNRPD3 Polyclonal antibody

SNRPD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10379-1-AP

Print datasheet

Synonyms

Sm D3, SMD3, snRNP core protein D3, SNRPD3

验证数据展示

  • WB analysis of MCF-7 using 10379-1-AP

    WB analysis of MCF-7 using 10379-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10379-1-AP (SNRPD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 10379-1-AP

    IHC staining of human small intestine using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with SNRPD3 antibody included
  • IHC staining of human small intestine using 10379-1-AP

    IHC staining of human small intestine using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 10379-1-AP (SNRPD3 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with SNRPD3 antibody included
  • IHC staining of human lung cancer using 10379-1-AP

    IHC staining of human lung cancer using 10379-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10379-1-AP (SNRPD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SNRPD3 antibody included

经过测试的应用

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IHC detected inhuman small intestine tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SNRPD3 Ready-To-Use IHC Kit. SNRPD3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10379-1-AP targets SNRPD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen SNRPD3 fusion protein Ag0293 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small nuclear ribonucleoprotein D3 polypeptide 18kDa
Synonyms Sm D3, SMD3, snRNP core protein D3, SNRPD3
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC000457
Gene Symbol SNRPD3
Gene ID (NCBI) 6634
RRIDAB_2286308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Small nuclear ribonucleoprotein D3 polypeptide 18kDa (SNRPD3,synonym: SMD) belongs to the small nuclear ribonucleoprotein core protein family, with molecular weights of the D members: D1,16 kDa; D2, 16.5 kDa and D3 18 kDa. SNRPD3 is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis.

实验方案

Product Specific Protocols
WB protocol for SNRPD3 antibody 10379-1-APDownload protocol
IHC protocol for SNRPD3 antibody 10379-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

CRISPR-based RNA-binding protein mapping in live cells.

Authors - Baiwen Chen
View Article
humanIHC

Transl Oncol

Identification of genes associated with local aggressiveness and metastatic behavior in soft tissue tumors.

Authors - Cunha Isabela Werneck IW
View Article