验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, rat heart tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13131-1-AP targets SNTA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | SNTA1 fusion protein Ag3820 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component) |
| 别名 | Alpha 1 syntrophin, SNT1, SNTA1, Syntrophin 1, TACIP1 |
| 计算分子量 | 505 aa, 54 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC026215 |
| 基因名称 | SNTA1 |
| Gene ID (NCBI) | 6640 |
| RRID | AB_3085408 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13424 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SNTA1, also known as Syntrophin 1, SNT1, is cytoplasmic peripheral membrane scaffold proteins that are components of the dystrophin-associated protein complex. SNTA1 is a signal-transducing adaptor protein and serves as a scaffold for various signaling molecules. Alpha-1 syntrophin contains a PDZ domain, two Pleckstrin homology domains, and a 'syntrophin unique' domain. SNTA1 is the high expression in skeletal muscle and heart (PMID: 8576247, 8612778,). The PDZ domain of SNTA1 has been reported to bind to the C-terminal domain of murine cardiac voltage-gated sodium channels (SkM2) causing altering ion channel activity leading to Long QT syndrome(PMID 19684871, 19862833).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SNTA1 antibody 13131-1-AP | Download protocol |
| IHC protocol for SNTA1 antibody 13131-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |