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验证数据展示

  • WB analysis of HeLa using 15358-1-AP

    WB analysis of HeLa using 15358-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15358-1-AP (SNUPN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 15358-1-AP

    IHC staining of human brain using 15358-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15358-1-AP (SNUPN antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 15358-1-AP

    IHC staining of human brain using 15358-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15358-1-AP (SNUPN antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of MCF-7 using 15358-1-AP

    IF Staining of MCF-7 using 15358-1-AP

    Immunofluorescent analysis of MCF-7 cells, using SNUPN antibody 15358-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SNUPN Polyclonal antibody

SNUPN Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15358-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Snurportin-1, Snurportin1, snurportin 1, RNUT1, RNA U transporter 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15358-1-AP targets SNUPN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 SNUPN fusion protein Ag7604 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 snurportin 1
别名 Snurportin-1, Snurportin1, snurportin 1, RNUT1, RNA U transporter 1
计算分子量 41 kDa
观测分子量 41 kDa
GenBank蛋白编号BC004203
基因名称 SNUPN
Gene ID (NCBI) 10073
RRIDAB_2192073
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95149
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SNUPN antibody 15358-1-APDownload protocol
IHC protocol for SNUPN antibody 15358-1-APDownload protocol
IF protocol for SNUPN antibody 15358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Nat Commun

SNUPN deficiency causes a recessive muscular dystrophy due to RNA mis-splicing and ECM dysregulation

Authors - Marwan Nashabat
View Article
humanWB

Neurobiol Dis

UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins.

Authors - Simona Rossi
View Article