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  • Featured Product
  • KD/KO Validated

SNW1 Polyclonal antibody

SNW1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25926-1-AP

Print datasheet

Synonyms

NCOA 62, SKIP, SKIIP, Ski-interacting protein, Nuclear receptor coactivator NCoA-62

验证数据展示

  • WB analysis of HepG2 using 25926-1-AP

    WB analysis of HepG2 using 25926-1-AP

    WB result of SNW1 antibody (25926-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNW1 transfected HepG2 cells.

  • IHC staining of human prostate cancer using 25926-1-AP

    IHC staining of human prostate cancer using 25926-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25926-1-AP (SNW1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 25926-1-AP

    IF Staining of HepG2 using 25926-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNW1 antibody (25926-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human prostate cancer using 25926-1-AP

    IHC staining of human prostate cancer using 25926-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25926-1-AP (SNW1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of MCF-7 using 25926-1-AP

    WB analysis of MCF-7 using 25926-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25926-1-AP (SNW1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25926-1-AP

    IF Staining of HepG2 using 25926-1-AP

    Immunofluorescent analysis of HepG2 cells using 25926-1-AP (SNW1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, HepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25926-1-AP targets SNW1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SNW1 fusion protein Ag23106 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SNW domain containing 1
Synonyms NCOA 62, SKIP, SKIIP, Ski-interacting protein, Nuclear receptor coactivator NCoA-62
Calculated Molecular Weight 536 aa, 61 kDa
Observed Molecular Weight 62-69 kDa
GenBank Accession NumberBC108903
Gene Symbol SNW1
Gene ID (NCBI) 22938
RRIDAB_2880298
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13573
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SNW1 antibody 25926-1-APDownload protocol
IHC protocol for SNW1 antibody 25926-1-APDownload protocol
IF protocol for SNW1 antibody 25926-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.

Authors - Shuai Hou
View Article
humanWB

FEBS Lett

Split-BioID: a proximity biotinylation assay for dimerization-dependent protein interactions.

Authors - Sofie De Munter
View Article
humanWB

Microbes Infect

SNW1 interacts with IKKγ to positively regulate antiviral innate immune responses against influenza A virus infection.

Authors - Qiao Zhang
  • KD Validated
View Article
mouseWB

J Ovarian Res

Alteration of the N6-methyladenosine methylation landscape in a mouse model of polycystic ovary syndrome

Authors - Lingxiao Zou
View Article