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验证数据展示

  • WB analysis using 26727-1-AP

    WB analysis using 26727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26727-1-AP (SNX10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 26727-1-AP

    IHC staining of human pancreas cancer using 26727-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26727-1-AP (SNX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 26727-1-AP

    IHC staining of human pancreas cancer using 26727-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26727-1-AP (SNX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SNX10 Polyclonal antibody

SNX10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26727-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Sorting nexin-10, sorting nexin 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26727-1-AP targets SNX10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SNX10 fusion protein Ag24977 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sorting nexin 10
Synonyms Sorting nexin-10, sorting nexin 10
Calculated Molecular Weight 201 aa, 24 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC034992
Gene Symbol SNX10
Gene ID (NCBI) 29887
RRIDAB_3085897
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5X0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins classified by the presence of a phospholipid-binding motif PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. Mutations in SNX10 (Sorting nexin-10) have been found to account for approximately 4% of all human autosomal recessive osteopetrosis (ARO) that is a genetically heterogeneous disorder caused by reduced bone resorption by osteoclasts (PMID: 23280965; PMID: 28592808).

实验方案

Product Specific Protocols
WB protocol for SNX10 antibody 26727-1-APDownload protocol
IHC protocol for SNX10 antibody 26727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols