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验证数据展示

  • WB analysis using 26727-1-AP

    WB analysis using 26727-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26727-1-AP (SNX10 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 26727-1-AP

    IHC staining of human pancreas cancer using 26727-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26727-1-AP (SNX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 26727-1-AP

    IHC staining of human pancreas cancer using 26727-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26727-1-AP (SNX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SNX10 Polyclonal antibody

SNX10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26727-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Sorting nexin-10, sorting nexin 10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26727-1-AP targets SNX10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SNX10 fusion protein Ag24977 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sorting nexin 10
别名 Sorting nexin-10, sorting nexin 10
计算分子量 201 aa, 24 kDa
观测分子量25 kDa
GenBank蛋白编号BC034992
基因名称 SNX10
Gene ID (NCBI) 29887
RRIDAB_3085897
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5X0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sorting nexins are a diverse group of cytoplasmic and membrane-associated proteins classified by the presence of a phospholipid-binding motif PX domain (PMID:12461558). They are involved in endocytosis and protein trafficking. Mutations in SNX10 (Sorting nexin-10) have been found to account for approximately 4% of all human autosomal recessive osteopetrosis (ARO) that is a genetically heterogeneous disorder caused by reduced bone resorption by osteoclasts (PMID: 23280965; PMID: 28592808).

实验方案

Product Specific Protocols
WB protocol for SNX10 antibody 26727-1-APDownload protocol
IHC protocol for SNX10 antibody 26727-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols